PEER-REVIEW | DERMATOLOGY | Figure 6 Epiluminescence
ultrastructure and sizes (A) pre- and (B)
post-treatment (T0 vs T120).
The examinations included clinical observation, a
number of different instrumental tests, and the patients’ own subjective considerations. The instrumental tests were: Photographic documentation (global photo) Pull test15, 16 Trichogram15, 16 Count of fallen hairs (wash-test carried out by the patient after washing her hair)15, 16
Dermatoscopic examination, for assessing the physical parameters and density of the hair3 performed by DermLite Foto system17
.
Product The product is a gel comprising highly purified PN of natural origin (Plinest® Fast, 7.5 mg/ml). It is supplied in pre-filled glass syringes, each containing 1.3 ml of high molecular weight, sterile, and apyrogenic PN. It is a biocompatible class III medical device (CE 0373) of natural origin and allergy testing is not necessary. The product has undergone many in vitro and in vivo tests18,19
, showing the
absence of local and systemic toxicity, in accordance with UNI standards for medical devices (cytotoxicity by direct contact, allergic sensitisation, Ames mutagenicity test, intracutaneous reactivity, irritation test). In clinical use, high tolerability and the total absence of serious side-effects have been reported. The PNs were kindly supplied free of charge (Plinest® Fast).
Results The treatment was carried out in accordance with the protocol on all the patients enrolled in the study. No local or systemic side-effects requiring suspension of the treatment were observed. The results of the treatment were, overall, highly
satisfactory, as shown in the tables provided, with a marked reduction in hair loss (Figures 1–4) alongside an increase in the number of and diameter of the hair shafts (Figures 5 and 6). Furthermore, the trichogram (Figure 2)
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values and the pull test (Figure 1) values of the hair returned to a relatively ‘normal’ state in all patients treated. As far as compliance is concerned, the patients
accepted the treatment willingly, also considering the clearly noticeable clinical results (Figure 7), as well as the objective data acquired.
Discussion Thellung et al11
and Sini et al8 demonstrated that PN
enhanced the proliferation of human fibroblasts in vitro. Belletti et al studied the effect of PN on UVB-damaged fibroblasts. The study showed that PN increased the rate of repair of UVB-damaged dermal fibroblasts20
and
appeared to promote the proliferation of human pre-adipocytes9
. A recent study evaluated the effect of
PN and hyaluronic acid (HA) on cultured human fibroblasts by analysing cell growth10
. The results
confirmed the activity of PN on human fibroblasts, while no significant stimulation was obtained with HA alone,
The results of the treatment
were, overall, highly satisfactory, as shown in the tables provided, with a marked reduction in hair loss alongside an increase in the
number of and diameter of the hair shafts.
demonstrating the trophometabolic action of polynucleotides in different cell lineage 10 PN are considered important bio-
.
stimulating agents in aesthetic medicine owing to their specific action on fibroblasts, as shown by a number of clinical studies investigating their use21–24
. With regard to the scalp, tests were
conducted in the past on patchy alopecia25
, and an initial test was
more recently conducted for the treatment of androgenic alopecia with PN (7.5 mg/ml)26
. This study
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