Volume 17
Number 5 October 2011 Dear Abbe table of contents preview
Analysis of Cultural Heritage Special Section Introduction to Anaylsis of Cultural Heritage Clive Walker and Ineke Joosten
A SEM-EDS Study of Cultural Heritage Objects with Interpretation of Constituents and Teir Distribution Using PARC Data Analysis Corrie J.G. van Hoek, Michiel de Roo, Grishja van der Veer, and Sieger R. van der Laan
Te External Ion Microbeam of the LABEC Laboratory in Florence: Some Applications to Cultural Heritage Silvia Calusi
Portable Apparatus for In Situ X-Ray Diffraction and Fluorescence Analyses of Artworks Myriam Eveno, Brice Moignard, and Jacques Castaing
PXRF, µ-XRF, Vacuum µ-XRF, and EPMA Analysis of “Email Champlevé” Objects Present in Belgian Museums Veerle Van der Linden, Eva Meesdom, Annemie Devos, Rita Van Dooren, Hans Nieuwdorp, Elsje Janssen, Sophie Balace, Bart Vekemans, Laszlo Vincze, and Koen Janssens
Quantitative Determination of van Gogh’s Painting Grounds Using SEM/EDX Ralph Haswell, Leslie Carlyle, and Kees T.J. Mensch
Multispectral Infrared Reflectography to Differentiate Features in Paintings Claudia Daffara and Raffaella Fontana
SEM Backscattered-Electron Images of Paint Cross Sections as Information Source for the Presence of the Lead White Pigment and Lead-Related Degradation and Migration Phenomena in Oil Paintings Claudia Daffara and Raffaella Fontana
Microanalysis Applications Study of Mural Paintings Using In Situ XRF, Confocal Synchrotron-µ-XRF, µ-XRD, Optical Microscopy, and SEM-EDS—Te Case of the Frescoes from Misericordia Church of Odemira S. Valadas, A. Candeias, J. Mirão, D. Tavares, J. Coroado, Rolf Simon, A.S. Silva, M. Gil, A. Guilherme, and M.L. Carvalho
Quantitative Energy Dispersive X-Ray (EDX) Analysis of Sub-Micrometric Particles Using a Scanning Electron Microscope (SEM) Luigi Paoletti, Biagio M. Bruni, Antonio Gianfagna, Simona Mazziotti-Tagliani, and Alessandro Pacella
An Examination of Kernite (Na2B4O6(OH)2 • 3H2O) Using X-Ray and Electron Spectroscopies: Quantitative Microanalysis of a Hydrated Low-Z Mineral Douglas C. Meier, Jeffrey M. Davis, and Edward P. Vicenzi
Materials Applications Comprehensive Comparison of Various Techniques for the Analysis of Elemental Distributions in Tin Films D. Abou-Ras, R. Caballero, C.-H. Fischer, C. Kaufmann, I. Lauermann, R. Mainz, H. Mönig, A. Schöpke, C. Stephan, C. Streeck, S. Schorr, A. Eicke, M. Döbeli, B. Gade, J. Hinrichs, T. Nunney, H. Dijkstra, V. Hoffmann, D. Klemm, V. Efimova, A. Bergmaier, G. Dollinger, T. Wirth, W. Unger, A.A. Rockett, A. Perez-Rodriguez, J. Alvarez-Garcia, V. Izquierdo-Roca, T. Schmid, P.-P. Choi, M. Müller, F. Bertram, J. Christen, H. Khatri, R.W. Collins, S. Marsillac, and I. Kötschau
Electron Image Series Reconstruction of Twin Interfaces in InP Superlattice Nanowires Martin Ek, Magnus T. Borgström, Lisa S. Karlsson, Crispin J.D. Hetherington, and L. Reine Wallenberg
Structure and Chemistry across Interfaces at Nanoscale of a Ge Quantum Well Embedded within Rare Earth Oxide Layers Tanmay Das and Somnath Bhattacharyya
Influence of Surfactant Concentration on the Surface Morphology of Hollow Silica Microspheres and Its Explanation Shiquan Liu, Meiying Wei, Pegie Cool, Cynthia Van Oers, and Jiancun Rao
Closed-Cell Foam Skin Tickness Measurement Using an SEM Clifford S. Todd and Valentina Kuznetsova
Biological Applications Quantification of Collagen Organization and Extracellular Matrix Factors within the Healing Ligament Connie S. Chamberlain, Erin M. Crowley, Hirohito Kobayashi, Kevin W. Eliceiri, and Ray Vanderby
Minerals and Aligned Collagen Fibrils in Tilapia Fish Scales: Structural Analysis Using Dark-Field and Energy-Filtered Transmission Electron Microscopy and Electron Tomography Mitsuhiro Okuda, Nobuhiro Ogawa, Masaki Takeguchi, Ayako Hashimoto, Motohiro Tagaya, Song Chen, Nobutaka Hanagata, and Toshiyuki Ikoma
A Novel Algorithm for the Determination of Bacterial Cell Volumes Tat is Unbiased by Cell Morphology M. Zeder, E. Kohler, L. Zeder, and J. Pernthaler
Equipment/Techniques Development Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy Marie Vancová, Miroslav Šlouf, Jan Langhans, Eva Pavlová, and Jana Nebesářová
Te Tree-Dimensional Point Spread Function of Aberration-Corrected Scanning Transmission Electron Microscopy Andrew R. Lupini and Niels de Jonge
A Piezoelectric Goniometer Inside a Transmission Electron Microscope Goniometer Wei Guan, Aiden Lockwood, Beverly J. Inkson, and Günter Möbus
High Contrast Magnetic and Nonmagnetic Sample Current Microscopy for Bulk and Transparent Samples Using Soſt X-Rays Daniela Nolle, Markus Weigand, Gisela Schütz, and Eberhard Goering
Book Review Te Image Processing Handbook John C. Ross
80
Dear Abbe, I am interested to hear your views on air-conditioning requirements for confocal microscope rooms. We have central air conditioning with temperature sensors in each microscope room, but this setup does not keep the temperature sufficiently stable. We are thinking of installing a dedicated unit similar to those used in computer rooms. Tis should maintain the temperature and control humidity, but prices for such units are very high. Anya in Hawkesbury
Dear Anya, Oy gevalt! What is this world coming to when we have to coddle these whiny namby-pambies who opine that “it’s too cold” or “it’s too hot” or “I have lost all feeling in my fingers and my core body temperature has dropped to dangerous levels—may I please come inside the lab?” Now we are extending these same courtesies to inanimate microscopes! When I used to build instruments we made them TOUGH! Te prototype of the Zeiss Ultraphot was built to withstand just about anything. Carl Z and I once took it out back and emptied an entire clip from my trusty Walther p38 toward it. We laughed heartily when the shots would ricochet off in all directions; at one point coming straight back and knocking my spectacles clear off my head. Imagine the fun we would have had if we had also been drinking?
Dear Abbe, I have a question about live imaging. We are planning on running an experiment where we want to follow a cell that will be moving quite a lot over an extended period of time. We need to keep this cell in the field of view throughout the whole experiment. Te two possibilities that come to mind are either taking a relatively large tilescan at every timepoint and hope that the cell will not go past the area, or writing a lengthy and complex macro in a program in order to detect the cell’s movement within the field and move the stage with it over time. Have you done a similar experiment? No Time in New York
Dear Timeless, For crying out loud, why all this “automated” nonsense? What is wrong with doing it the way we did in the old country, namely strapping an undergraduate to a chair and making THEM follow the blasted cell? If you keep feeding him or her Twinkies and Red Bull, they should have no problem doing this for at least two days. If one provides a waste receptacle capable of containing liquids, this can be extended indefinitely. It is a scientific rite-of-passage to be stuck watching over cells or reactions for interminably long periods. Most advisors don’t really need the data—think of it as benign scientific hazing. Developing the skills required can be useful in future situations. I once spent an entire week staking out the apartment of Frau Ingrid Grosse Brüste and did not doze off once (although I did have to pretend to be a passed-out landstreicher when her husband came over to inspect the bushes where I was hiding).
Even if you’ve already consulted your Scientific Ouija board, you still need to get that second opinion! Write to Professor Abbe through his personal associate administrative assistant at
jpshield@uga.edu.
doi:10.1017/S1551929511000952
www.microscopy-today.com • 2011 September
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84