Pluripotent Stem Cell Technology
Recent advances in stem cell technology utilize repeated mRNA transfection as a faster and more effi cient method to generate induced pluripotent stem cells (iPSCs) compared to DNA transfection, viral, or protein transduction.
Find out more in the following articles highlighting TransIT® in RiPSC Technology:
-mRNA Transfection Kit
• Angel M, Yanik MF. 2010. Innate Immune Suppression Enables Frequent Transfection with RNA Encoding Reprogramming Proteins. PLoS One. Jul 23;5(7):e11756.
• Warren L, et al. 2010. Highly Effi cient Reprogramming to Pluripotency and Directed Differentia- tion of Human Cells with Synthetic Modifi ed mRNA. Cell Stem Cell. Volume 7, Issue 5, 618-630.
100 120
20 40 60 80
0 A549 To inquire about the TransIT® -mRNA Transfection Kit,
Visit
www.TheTransfectionExperts.com E-mail:
techsupport@mirusbio.com or call 888.530.0801 (U.S. only); +1.608.441.2852 (outside the U.S.)
www.TheTransfectionExperts.com More than a decade of gene delivery expertise
©2011 All rights reserved Mirus Bio LLC. All trademarks are the property of their respective owners. TransIT® CHO-K1 COS-7 HEK293 HeLa NIH3T3 Vero
Cells. Cells were transfected at 80% confl uency with a capped, poly-A mRNA encoding fi refl y lu- ciferase using either TransIT-mRNA (Mirus Bio) or TransMessenger®
-mRNA Transfection Kit Provides High Effi ciency Transfection of Large RNA into Mammalian (Qiagen). Luciferase activity
(solid bars) was assayed 18 hours post-transfection and normalized against values obtained using TransIT-mRNA. Visualized cell confl uency at the time of harvest (black diamonds) was 100% using TransIT-mRNA compared to lower values using TransMessenger Reagent.
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Validated RNA Induced
TransIT®
©2010 Mirus Bio LLC. All trademarks are the property of their respective owners. All rights reserved. -mRNA Reagent TransMessenger® Reagent Cell Confluency
Normalized Luciferase Activity
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