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high performance immunoassays InstantOne™ ELISA


The evolution of phospho ELISAs leaps forward with the effi cient sensitivity and fl exibility of InstantOne ELISAs.


• Fast: Less than 1/3 the time of traditional ELISAs • Easy: 1/4 the eff ort with only 1 wash step


• Flexible: Enables the detection of total and/or phospho protein levels of a single protein up or down a pathway on a single plate


• Sensitive: Meeting or exceeding industry standards with only 2-3,000 cells needed


• Compatible: Colorimetric detection; utilizes standard plate readers • Complete: Over 30 kits available, with more coming soon


20 10 5 2.5 1.25 0.63 [HEK 293 lysate] µg protein/lane HEK cells are treated with 20% FBS for 15 min prior to lysis. Visit eBioscience.com/ddw-instantone for new kits to fast forward your research.


ORDER 888.999.1371 • TECH 888.810.6168 • INQUIRIES info@eBioscience.com • WEB www.eBioscience.com For Research Use Only. Not for use in diagnostic or therapeutic procedures.


0.31 4 3 2 1 0 [HEK 293 lysate] µg protein/well phospho-AKT (Ser473)


Absorbance (450nm)


20 10 5 2.5 1.25 0.625 0.3125


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