11-02/03 :: February / March 2011
nanotimes News in Brief
François Boily and Madeleine Ramstedt, were investigating whether new physiochemical analysis methods could also be used for microbial studies. The scientists combined so-called cryo-x-ray pho- toelectron spectroscopy with multivariate analysis. This analysis yields specific patterns of intensity curves depending on the chemical composition of the surface of the material being studied.
“We’ve succeeded also in analyzing the cell sur- faces of bacteria with our x-ray spectroscopy. We found strong patterns that we could clearly relate to different compositions in lipids, sugar, protein, and the polymer peptidoglycan in the cell wall of the bacterium that can affect the capacity of a bacte- rium to infect an organism,” explains Madeleine Ramstedt. “The method makes it possible to analyze the outermost layer, about 10nm from the surface.”
Madeleine Ramstedt, Ryoma Nakao, Sun Nyunt Wai, Bernt Eric Uhlin, and Jean-Francois Boily: Monitoring sur- face chemistry changes in the bacterial cell wall – multi- variate analysis of Cryo-X-ray photoelectron spectroscopy data, In: The Journal of Biological Chemistry, February 17, 2011, DOI:10.1074/jbc.M110.209536:
http://dx.doi.org/10.1074/jbc.M110.209536
69
technique, capable of detecting even single mole- cules, after decades of waiting in specialized labo- ratories has finally got a chance to widespread and revolutionize medical diagnostics.
SERS substrates designed by Polish researchers have unique properties. „The substrates commercially available so far had to be handled with extreme caution. They had to be stored under nitrogen, could not be touched and even though they were loosing their enhancement ability within hours only. Our substrates can be put into an ordinary drawer for a couple of months and they will be still usable” – stresses Ph.D. Agnieszka Michota- Kaminska from the IPS PAS. For economic reasons, it is important that Polish substrates are suitable for multiple use as the only ones worldwide. Their sur- face structure is so stable that the researchers from the IPC PAS were able to develop efficient cleaning procedures to assure that a high level of SERS signal enhancement is maintained.
The substrates for SERS studies have been develo- ped under the project „Quantum semiconductor nanostructures for applications in biology and medicine”.
Gold-coated substrates of gallium nitride with spe- cifically formed surface, developed by researchers from the Institute of Physical Chemistry of the Polish Academy of Sciences and the Institute of High Pressure Physics of the PAS display world- wide unique properties. Thanks to these new substrates, an extremely sensitive SERS analytical
One of the project’s long term goals is to develop a sensor for detection of antibodies in blood. The core element of the device will be a semiconductor with a surface network of thousands of micrometer size wells.
http://www.ichf.edu.pl
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95