Epigenetics


The Biocius (www.biocius.com) RapidFire label- free mass spectrometry platform enables high throughput analysis of a wide range of epigenetic target enzymes. The system is uniquely capable of detecting multiple modification events across a sin- gle, native substrate; a challenging endeavour with other technologies using optically or radioactively labelled surrogate substrates. For example, lysine can accommodate up to three methyl groups, thus a variety of methylated states are possible depend- ing on the activity of methylases and demethylases acting upon the protein. RapidFire mass spectrom- etry can detect and distinguish any of the methyla- tion patterns: unmethylated, single, di- or tri- methylated. The RapidFire platform has been used to assess the activity of a variety of epigenetic enzymes, including protein acetylases/deacetylases, protein methylases/demethylases and DNA methy- lases/demethylases. Recent RapidFire data even demonstrates the simultaneous monitoring of SIRT-1 mediated deacetylation at several sites in the p53 gene sequence. Additionally, RapidFire technology demonstrates much faster analysis


Figure 11: Sequential deacetylation of a triply-acetylated p53 peptide by SIRT1 using Biocius RapidFire


Address Even the Most Di cult Assays RapidFireTM enables label-free


detec on of na ve analytes for previously intractable targets


Label-free screening for:


• oncology • epigene cs • metabolic disorders • cardiovascular disease • an -infec ves • neurological disorders • infl amma on


T: 781.928.2700 | F: 781.998.0054 | www.BIOCIUS.com | info@BIOCIUS.com Drug Discovery World Spring 2011 45


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