Epigenetics
Figure 10
ECL detection of Active Motif’s MODified Histone
Peptide Arrays treated with HMT G9a. MODified histone peptide Arrays were treated with: A) 25 M G9a
methyltransferase; B) 25 M G9a mutant H904K; C) no enzyme control, overnight in the presence of 1 mM
AdoMet. The arrays were detected using a Histone H3 dimethyl Lys9 antibody. Novel methylation sites were
observed on array A, which was treated with wild-type G9a histone methyltransferase, showing the activity of the histone modifying enzyme on the peptide substrate
HAT, HDAC and Histone demethylase. It pro- vides a wide selection of antibodies and recombi- nant proteins to allow researchers to develop their own screening assays. Using a proprietary tech- nique, Active Motif has generated full length recombinant histones to study site- and degree- specific methylation as well as acetylation modifi- cations. Its offering of recombinant proteins also includes a variety of histone modifying enzymes. Since it is known that some histone modifying enzymes prefer nucleosomes as a substrate over purified histones Active Motif now offers hela mononucleosomes for use in screening assays. Many important regulators of genome function contain protein motifs that interact with specific histone modifications. To facilitate the study of these proteins, it offers its MODified™ histone peptide array for analysis of up to four different modification combinations within the same pep- tide sequence. The peptide arrays are also useful tools for validating the specificity of Active Motif’s expanding line-up of antibodies to histone modifications (Figure 10).
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