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Application Note


Automate sterile liquid handling with the flexible FeliX


T


he FeliX liquid handling robot is the system of choice for cell han- dling and cell-based assays. The air


bench version has a small 17” x 25” foot- print that allows it to fit nearly any class 2 clean air hood. The robot has a unique two-level deck with 12 positions and an open area to the back and front that is designed to allow even airflow through and around the robot. It is resistant to alcohol and UV light decontamination. Boxed ster- ile filter tips are available for 1,000µl down to 0.5µl dispensing. Capillary and pin tool accessories are available for the nL volume ranges. Alcohol tip wash and blotting sta- tions are available for these nL volume ranges. The Felix robot has very precise x/y and z control and can process single, 8-, 12-, 16-, 24-, 96-, 384- and 1536-well for- mats. Mixing, aspiration and dispense speeds can be set as low as 8µl/second for delicate cells. The on deck gripper can stack up to five standard well plates on the bottom deck and three standard well plates on the top deck. The gripper can also place and remove plate lids. A temperature con- trolled bio-shaker is available for source reservoirs during cell seeding applications.


A 4˚C cooled plate is also available for tem- perature sensitive reagents.


Who puts robots in hoods? We do. Put the CyBio FeliX into your own closure. The dimensions of the FeliX robot are 17” x 24” x 24” which makes it one of the smallest automated liquid handling sys- tems on the market, shown in Figure 1.


Keeping it sterile l Felix available with a UV decontamina- tion light. l Felix open back and front design for laminar air flow. l Sterile boxed filter tips in all sizes 60µl, 250µl, 1,000µl. l All components can be sprayed down with 70% EtOH.


CyBio FeliX: cell-based applications lCell seeding (gentle slow aspiration/dispense). lCell-based assays (serial dilutions).


lRe-arraying: 96- to 24-well (6-channel adaptor). l Re-arraying: 96- to 384-well (96-channel head). l Cherry picking: 96- to 24- or 96-well (8- channel SELECT head). l Sub-culturing: 24- to 24-well (6-channel head, timed digestion step). Options for cell media reservoirs are as


follows; disposable reservoirs for single- channel, row or column, rack system to hold strip reservoirs (maximum 2 x 100mL or 4 x 30mL per rack), reservoirs for 96- and 384-channel, and automatic refill reser- voirs with level tracking (volume approxi- mately 100mL).


Interface: easy-to-use software solutions CyBio Application Studio l Ready to use user interface. l Click and run. CyBio Composer l Flexible automation software. lOpen remote control and driver interfaces. CyBio Overture l Easy-to-use instrument control software. l Superior liquid handling support.


Figure 1: Clean bench FeliX Drug Discovery World Winter 2019/20


Conclusion The CyBio FeliX is an ideal flexible liquid handling platform to improve reproducibil- ity of the results and reduce the hands-on time. The liquid handler allows test com- pounds to be serially titrated and applied to cells; it is a flexible, competitively-priced instrument capable of conducting neces- sary assay set-up and reagent delivery steps of a profiling workflow. Incorporating thaw and use cells provides a reproducible source of cells and removes the burden of dedicating time to cell culture, and using the CyBio FeliX liquid handler removes the burdensome and more variable manual liq- uid handling. Such an automated workflow provides researchers with walk-away con- venience while generating consistent and reproducible results.


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