Screening


Table 1: Drug screening using organoids10 CANCER TYPE Colorectal


ORGANOID TYPE


CSC-derived LIBRARY


Target-known inhibitors + chemo drugs


Breast CSC-derived


EGFR/AKT/mTORC pathway inhibitors


Gastric Bladder CSC-derived CSC-derived


Approved anti-cancer drugs


Target-known inhibitors + chemo drugs


Liver CSC-derived


NCI-approved oncology drugs set VIII


Various CSC-derived


Chemo drugs and targeted agents under clinical development


Ovarian CSC-derived


Target-known inhibitors + chemo drugs


Colorectal CSC-derived*


Target-known inhibitors + chemo drugs


Endometrial CTOS


Target-known inhibitors


Colorectal Colorectal CTOS CTOS


Target-known inhibitors


Target-known inhibitors + FDA-approved drugs


# COMPOUNDS TESTED


83 19


# CASES TESTED


ASSAY CONDITIONS


With 2% BME in culture medium on BME


6 28


With 2% BME in culture medium on BME


37 50 7 11 On 50% Matrigel


With 2% Matrigel in culture medium


129 5 In Matrigel


160 (single) + 120 (combination)


22


4


2D culture of organoids for screening


10


With 2% Matrigel in culture medium on Matrigel


8 19 In Matrigel


79


5 (2 hit drugs evaluated in 12 CTOS lines)


71 2427 1


2 (15 hit drugs evaluated in 30 CTOS lines)


* Prepared by CTOS method; CSC = cancer stem cells; CTOS = cancer tissue-originated spheroid; BME = basement membrane extract


Without matrix


Without matrix Without matrix


Drug Discovery World Fall 2019


39


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