26 Preparing Samples of Cannabis Plant for THC/CBD Analysis
Accurate determination of the THC/CBD ratio is critically determined by effective sample preparation of the samples of cannabis leaf, bud and flower. Traditional sample preparation methods have used bead-beating or grinding mills to homogenise these materials. When conducted in polypropylene microplates the extreme forces applied by these machines often cause damage to the plates leading to cracking, leaking and ultimately, cross- contamination.
Porvair Sciences has applied modern design concepts and mould analysis to this problem to create a solid, stable and strong microplate that can withstand the applied forces in bead-beaters whilst maintaining a standard ANSI/SLAS footprint and key dimensions. By adding reinforced walls to the bottom of the well and backing these with supporting ribs on the underside of the plate, Porvair Sciences has produced a 2 ml square well block (Plant Genomics plate) that can be used safely and with confidence for Cannabis plant extraction. The Plant Genomics plate has a generous working volume of 1.90 ml per well in a 45mm high plate. The polypropylene used to mould the plate is pure, virgin, extractable free polymer, rigorously tested for leachates thereby ensuring no contamination from the plate will affect your THC/CBD analysis.
In the described protocol, sample preparation using the Plant Genomics plate in a GenoGrinder takes just 2 minutes. Following this, the dry ground Cannabis powder is transferred into the wells of a Porvair P3 filter microplate for extraction under agitation with methanol or isopropanol to dissolve the oily residues in the plant material. After agitation, the P3 plate is placed onto a Porvair Microlute manifold and vacuum applied to draw the purified extract down through the filter and into the wells of a collection plate. The collection plate containing the organic extract is then taken for HPLC analysis to determine the THC/CBD ratio. To determine total THC and CBD in the Cannabis plant sample, the purified extract should be gently evaporated to dryness using a Porvair MiniVap blowdown evaporator. The sample should then be redissolved in a known volume of solvent ready for HPLC analysis. Both methodologies are reported to be straightforward and repeatable.
More information online:
ilmt.co/PL/lyRz 48294pr@reply-direct.com
Why Should I Use the VPHP Method? H2
O2
(hydrogen peroxide) is an oxidising agent which, when it comes into contact with micro-organisms, oxidises their cells or spores, thus deactivating them. It is however, a surface decontaminant so may not penetrate dirt and detritus; heavily soiled surfaces should be thoroughly cleaned prior to decontamination. This system is operated at ambient room temperature and relative humidity, without the need to significantly reduce humidity, which was traditionally required for fumigation bio-decontamination.
There is now a large body of published scientific research demonstrating that hydrogen peroxide vapour technology is able to inactivate bacteria, viruses and fungi, as well as reduce healthcare associated infections (HAIs), giving you assurance of a 6-log spore reduction.
Not only is VPHP/ VHP proven to be an effective decontaminator, it is a more rapid and convenient means of fumigation, reducing your equipment downtime, and is significantly less hazardous to health than traditional fumigation methods. Equipment and rooms which are subject to regular health and safety testing and validation, or need regular servicing should be decontaminated appropriately prior to these activities.
Many customers perform the fumigation themselves before a service, however, BPS Crowthorne, by encapsulating the expertise of sister company Crowthorne Hi-Tec Services, can save your time with an equipment bio-decontamination service (EBDS) and perform the fumigation prior to service ensuring a full and successful service with minimal disruption and downtime. BPS Crowthorne can also fumigate and service several cabinets at the same time. Not only does the EBDS service save you time, it also saves you the capital outlay of buying your own equipment.
Want to know more about EBDS or any of our other services and products? Get in touch with BPS Crowthorne to discuss your requirements today.
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Highly Reproducible and Sustainable Low Temperature Reactions and Studies
The DrySyn SnowStorm range from Asynt offers an attractive solution for scientists looking to undertake low temperature chemical reactions and studies. Unlike traditional reaction cooling methods, including ice, dry-ice and liquid nitrogen; accurate and prolonged temperature control on a DrySyn SnowStorm is regulated by an external thermostatic circulator. The use of such a temperature control system not only eliminates the risk of temperature fluctuations due to perishable coolants, but can also improve your labs sustainable use of resources.
In addition to allowing unattended operation (even overnight), active temperature control on the DrySyn Snowstorm range also enables responsive control of exothermic reactions, constant rate cooling to enhance polymorph studies and significant improvement in reaction reproducibility. Beneficially the ability to agitate and cool in parallel using a DrySyn Snowstorm can also lead to improved productivity.
The DrySyn Snowstorm systems deliver precise temperature control of reactions from -60 to +150°C and have been designed to be user friendly and practical, with a small bench top footprint. The DrySyn SnowStorm ONE enables controlled chemistry to be conducted on single flasks up to 1 litre in size, whilst the SnowStorm MULTI allows for up to three 100 ml parallel reactions to be undertaken. The use of a weighted silicone seal prevents the formation of ice around the glassware on these systems. For smaller scale experiments, the DrySyn Snowstorm Reactor allows increased throughput and screening of up to 27 vial-based reactions. Purging the dome with nitrogen / argon prevents the formation of ice around the vials at sub-ambient temperatures.
More information online:
ilmt.co/PL/vXnA 48520pr@reply-direct.com
Enhanced Connectivity and Data Management Enables Efficiency of Weighing Processes to be Optimised
Mettler Toledo is pleased to announce an upgrade to their MS-TS advanced level analytical and precision balances, which provides advanced connectivity and reporting capabilities for easier data management.
MS-TS advanced level balances are equipped with four interfaces and various services for easy connectivity. With the choice of built-in Ethernet, USB Host, USB Device and RS232 interfaces, it is quick and easy to connect MS-TS Advanced Level balances to peripheral devices and company networks (such as LAN, PC, USB memory stick, printer, barcode reader, keyboard or auxiliary display). New functionality allows data transfer directly to an FTP server. With the additional options of WLAN and Bluetooth, via the new wireless dongle, transfer of weighing data is simple and convenient. Features such as Balance Web Access enable remote viewing of weighing results, by accessing the MS-TS balance from a web browser on any tablet or PC. This makes it possible to view weighing results from anywhere within your company network, as well as perform basic balance operations such as tare or print, from a second screen.
Advanced reporting and publishing options for MS-TS advanced level balances offer flexibility on how to save, print and export weighing results and associated metadata. Reports can be tailored to the user or process to further improve productivity whilst controlling product quality. Operators can customise the inclusion of weighing metadata to results reports according to their requirements. For example, information about balance setup, including the levelling status and the last adjustment, can be appended to support the accuracy of results. A variety of formats are available with the latest software version: PDF, TXT, XML, and CSV. This allows weighing data to be transferred to a file server as a PDF report, which is ideally suited to on-screen viewing or electronic archiving. Alternatively, for further processing and analysis, data can be conveniently saved in standard CSV or XML file formats, either on to a USB memory stick or directly exported to an FTP server. Simultaneous publishing of different formats over different interfaces is also possible. For example, the balance can simultaneously send measurement reports to a strip printer via USB, for archiving in a lab journal, and send more comprehensive data files directly to a networked printer or FTP server via Ethernet.
More information online:
ilmt.co/PL/nOr9 and
ilmt.co/PL/33ow 48718pr@reply-direct.com
LABMATE UK & IRELAND - APRIL 2019
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