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Rapidly Standardise and Scale Your ChIP Workflows Chromatrap®


has entered into a partnership with Covaris to offer a highly scalable and simple ChIP workflow enabling researchers to easily


isolate protein-DNA complexes for downstream applications, such as ChIP-Seq, ChIP-qPCR, Next-generation sequencing (NGS), and Mass Spectrometry (MS).


Preparing samples for Chromatin Immunoprecipitation (ChIP) has traditionally been a multi-step, time-consuming, and error-prone workflow. Pairing the bead-free Chromatrap immunoprecipitation system with the Covaris truChIP®


and AFA® sample preparation technologies enables


investigators to rapidly profile protein-DNA interactions of interest. By combining the two technologies, researchers now have access to a compatible and optimised workflow that increases productivity and allows the user to gain better results with higher quality samples.


Chromatrap, the revolutionary automation-compatible spin column-based approach eliminates many of the challenges encountered when working with magnetic beads. This rapid centrifugation-based protocol requires only 3 simple washes using individual spin columns or 96-well plates and can be completed in a single day. In contrast, typical bead-based workflows take around 10 hours with over 10 wash steps and incubations.


Using the Covaris Adaptive Focused Acoustics® (AFA® ) technology, researchers can easily isolate and reproducibly shear soluble chromatin


without disrupting the protein-DNA interactions for immunoprecipitation with bead-free Chromatrap Protein A and G ChIP kits. More information online: bit.ly/2HAO3zP


48349pr@reply-direct.com


Extended Spectrum of Applications for Centrifugation Introduced


Eppendorf has introduced the high-capacity fixed-angle Rotor FA-6x250 for up to 6 x 250 mL bottles. The Rotor FA-6x250 extends the application range of the large benchtop Centrifuges 5910 R and 5920 R, which encompasses harvesting of bacteria, algae and yeast, as well as mammalian cells, in vessels up to 250 mL. An additional area of application now includes plasmid DNA extractions and lysate clearance following cell lysis.


A broad selection of 12 different adapters minimises the need for cumbersome rotor exchanges during the workflow; numerous vessel types of various volumes can now be accommodated by simply switching adapters. This simplifies handling and saves time.


The Eppendorf Centrifuges 5910 R and 5920 R also offer a large variety of rotors, thus serving countless applications in vessels ranging from 0.5 mL to 1,000 mL in volume. In the past, the use of 250 mL flat bottom bottles had been limited to swing-bucket rotors with a maximum speed just over 5,000 x g. The new fixed-angle rotor FA-6x250 now delivers a speed of up to 15,054 x g, effectively extending the range of application of the large Eppendorf benchtop centrifuges.


More information online: ilmt.co/PL/R4A4 48482pr@reply-direct.com


Entry Level Flow Reactors for Synthetic Development


Asynt announces their exciting new flow-chemistry platform, the fReactor, an affordable device created to bring the many advantages of flow chemistry to your laboratory through easy-to-use, flexible and intuitive design.


Developed in collaboration with chemists and chemical engineers from the renowned Institute of Process Research and Development (iPRD) at the University of Leeds, the fReactor is simple to assemble and modify, making it suitable for a wide range of continuous-flow processes.


Professor John Blacker, Head of the iPRD commented: “Our continuous stirred tank reactor design overcomes the limitations of tubular flow chemistry systems, most notably the fReactor allows both single and multiphasic reactions to be carried out across a range of residence times.” He added: “With the fReactor, just add a hotplate and pump and you are ready to start investigating flow chemistry experiments. Because of its low price and total operating volume of just 10ml, the fReactor is an ideal device for laboratories wanting to evaluate the many advantages of small- scale flow chemistry.”


Integrating the efficiency of pipe-flow processing with the advanced mixing of a continuous stirred tank reactor, the fReactor provides chemists with a versatile ‘plug-and-play’ setup allowing exploration of continuous-flow processing, with little expertise required.


The fReactor platform comprises of 5 modules, which combine to give a reaction zone delivering a good residence time distribution. These interconnected modules are located on a metal heat-transfer baseplate which sits on conventional laboratory hotplate-stirrer. Nikil Kapur, Professor of Applied Fluid Mechanics based at the School of Mechanical Engineering, commented: “With mixing in each zone, the fReactor offers outstanding flow chemistry reaction flexibility. Multiple ports allow telescoping of reactions, sampling or integration of sensors within the reactors. Robustly constructed, the fReactor is both easy to use and simple to clean.”


More information online: ilmt.co/PL/AL6B 48518pr@reply-direct.com


Cell-Free DNA Isolation Kit Launched Through Exclusive Partnership Beckman Coulter Life Sciences has launched the Apostle MiniMaxTM


High Efficiency Cell-Free DNA Isolation Kit through an exclusive partnership with Apostle, a liquid biopsy company. As cfDNA workflows


move beyond theoretical and into human health applications, Apostle’s industry-leading chemistry paired with Beckman Coulter Life Sciences’ portfolio of genomic reagents and liquid handling platforms enables researchers to meet the growing demands for sequencing of tumour-derived cfDNA.


Surpassing industry standards for yield and purity of cfDNA, Apostle MiniMax is a magnetic nanoparticle-based kit to extract cfDNA from plasma using manual or automated workflows. Due to limited cfDNA concentration, sufficient yield for next-generation sequencing (NGS) or other downstream applications can require higher volumes of plasma. As the volume increases, issues such as recovery efficiency and workflow complexity often arise. Plasma contains a host of contaminants that are problematic at this scale and can reduce assay sensitivity. Apostle MiniMax technology performs reliably across a range of volume inputs, consistently recovering high quantities of cfDNA, while effectively removing contaminants.


“Beckman Coulter Life Sciences is committed to working collaboratively with our customers to find the best technologies to meet their complex needs,” said Steve Wowk, Director of Genomics, Beckman Coulter Life Sciences.


“We have listened to their ongoing challenges in working with cfDNA and sought to find ways to effectively combat those issues. This includes recognising that an innovative product from a startup like Apostle is the best technology to address these needs.”


“Beckman Coulter Life Sciences’ established global presence and commitment to life sciences makes them an ideal collaborator to provide access to our innovative nucleic acid isolation products to customers around the globe,” said David Ge, President and CEO, Apostle Inc.


More information online: ilmt.co/PL/QGoP 48653pr@reply-direct.com


LABMATE UK & IRELAND - APRIL 2019


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