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UniFlow SE AireStream Laboratory Fume Hoods Unitized Superstructure for total chemical resistance Full line of Laboratory Cabinets & Epoxy Worksurfaces
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SALES@INTLABMATE.COM
Optimising cryopreservation for cardiovascular research
AMSBIO, in collaboration with the University of Strathclyde, announces a new poster detailing an extensive evaluation of the impact of three cryopreservation reagents (CELLBANKER® regarding viability and proliferative capacity.
1, CELLBANKER® 2, and commonly used 10% DMSO) on vascular smooth muscle cells (vSMCs)
Understanding vascular remodelling in cardiovascular diseases necessitates precise single-cell analysis of vSMCs. While large-scale solutions for single-cell analysis are well-established, challenges arise when working with freshly isolated vSMCs due to their rapid deterioration and loss of native phenotype. To enhance viable cell numbers and reduce animal use, a robust cryopreservation protocol for native vSMCs becomes essential.
The poster elaborates on Dr Mairi Sandison and Dr Michele Zagnoni’s innovative single-cell culture approach for tracking phenotypic heterogeneity in vSMCs, to advance our understanding of the cellular sub-populations that are drivers of disease. Their work, alongside doctoral student Ellis Smith, developed a sophisticated microwell array designed for the long-term confi nement of single vSMC progeny. This approach leverages the exceptional cell-repellent properties of Lipidure®
enabling reliable tracking of phenotypic changes in large numbers of single cells and their progeny over time. using Lipidure® material to confi ne single vSMC progeny long-term. CELLBANKER®
Key fi ndings highlight the remarkable advantages of CELLBANKER®
Download and read the poster in full:
ilmt.co/PL/dLV9 The CELLBANKER®
-CM as a coating material, to ensure robust confi nement of these highly adherent, migratory cells, -CM as a coating
cryopreservation media were utilised to preserve freshly isolated vSMCs, allowing increased experimentation from a single tissue. cryopreservation media. CELLBANKER®
1 signifi cantly improved survival rates of freshly isolated myocytes, maintaining high viability
(95%) post-thaw. In comparison, DMSO-based media showed lower post-thaw viability (78%). Furthermore, CELLBANKER 1&2 outperformed DMSO-based protocols in assessing frozen primary cell growth, displaying four to fi ve times greater cell growth after one week of culture.
series of cell freezing media enables the stable long-term storage of cells. With its unique formulation which enables stable cryopreservation and high viability after freeze-thaw procedures,
CELLBANKER is a trusted solution for the storage of any cell type including sensitive cell lines. More information online:
ilmt.co/PL/0QKk
61278pr@reply-direct.com Vacuum fi ltration systems with piston pumps
Wiggens vacuum fi ltration systems allows easier, faster, safer and more precise through the use in suspended solids measurement, HPLC, gas chromatography, AA micro-analysis, mobile phase decontamination, food and beverages industry, Pharma industry. Filtration has become a fundamental step of almost any chemical and life science research today.
Wiggens is proud to offer a truly stunning portfolio of fi ltration devices, all designed to maximise fi ltration effi ciency, yet also be cost effective and an easy fi ltration solution for the researcher and laboratory. The V series vacuum pump is a piston-powered, oil-free pump. With innovative electronic, mechanical technology and human design concept make, compact and lightweight, clean and maintenance free, safe and comfortable, it is very suitable to be used together with fi ltration system.
The many different kinds of vacuum fi ltration systems in this product line includes: All-In-One Filtration System; Filtration Collocations; Multi-Position Filtration Manifolds; Customer Assembling Options for Filtration System; Oil-free Piston vacuum pumps; Accessories For Filtration Systems.
For users processing many samples each day, Wiggens fi ltration solutions will help you reduce time, effort and waste. More information online:
ilmt.co/PL/L2gZ and
ilmt.co/PL/VEqd
58606pr@reply-direct.com
Index of Advertisers Anton Paar GmbH
DKK-TOA Corporation Integra Biosciences AG
16, OBC 23 15
Myron L Company Oxford Instruments Priorclave Ltd
29 9
OFC
Trans Instruments (TTBH Pte Ltd) VICI AG
31 5
Wiggens Technology (Beijing) Co, Ltd 17
LAB ASIA - FEBRUARY 2024
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