45
improved using another new stationary phase GreenSep NP-9. The chromatogram with enhanced separation between CBD and THC is shown in Figure 8. Using GreenSep NP-9 provides separation factor to effectively remove THC from a complex mixture of cannabinoids.
Isolation of CBD, THCV and THC
Figure 5: Separation of CBD, THC and CBN on GreenSep III using 2% ethanol.
The cannabinoid THCV is another cannabinoid that has some medicinal interest. However it is difficult to separate THCV from THC and CBD by SFC. However, the SFC isolation of THCV from CBD and THC was achieved on GreenSep NP-12. A chromatogram of this separation is shown in Figure 9.
Conclusion Figure 6: Cannabinoid mixture chromatographed on GreenSep NP-II using 10% ethanol.
Several new stationary phases have been developed (GreenSep NP-III, GreenSep NP-II, GreenSep NP-9 and GreenSep NP-12) optimised for the preparative SFC separation and isolation of cannabinoids. GreenSep NP-III is optimised for the rapid separation of CDBA and THCA. GreenSep NP-II is useful for THC and THCA removal with a quick cycle time. GreenSep NP-9 is optimised to deliver the maximum separation alpha between CBD and THC and is best for the removal of THC. GreenSep NP-12 is designed to separate CBD, THCV and THC with maximum alpha value. The recommended use for each of these stationary phases are shown in Table 1. Loading studies are currently being conducted to define preparative loading and output for these cannabinoid isolates. These stationary phases separate the desired components and are designed to deliver the desired separation at the lowest level of liquid ethanol modifier possible. Ethanol minimisation is important since it is more expensive than CO2 difficult to remove than CO2
and more . In addition, these Figure 7: Separation of CBD, THC and CBN on GreenSep III using 2% ethanol. Isolation of CBD and THC
During our investigation we discovered that GreenSep NP-III could be used for the SFC preparative separation of CBD and THC as shown in Figure 5 where both CBD and THC are eluted with only 2% ethanol.
The separation of CDB and THC on GreenSep NP-III provided the motivation to develop other new products specifically designed for optimised SFC preparative separation of cannabinoids. A chromatogram showing the separation of a cannabinoids mixture chromatographed on a GreenSep NP-II is shown in Figure 6 where
THC can easily be removed from a cannabis extract and CBDA and THCA are still eluted in less than 15 minutes.
In some cases, it would be desirable to isolate full spectrum CBD without THC. Full spectrum CBD contains cannabinoids without THC and THCA. This full spectrum CBD may have additional therapeutic benefits when compared to pure CBD. Figure 7 shows the separation of CBD and THC. Based upon this chromatography THC and CBN can be removed from an extract to produce full spectrum CBD.
The separation of CBD from THC was further
stationary phases are robust, cost effective and designed for preparative SFC separations.
Table 1: Recommended Cannabis Component Isolation for the New GreenSep NP Stationary Phases.
GreenSep Column Recommended Use NP-II
NP-III NP-9 NP-12
Rapid isolation of CBDA and THCA
Optimal THC removal to produce full spectrum CBD
Optimal separation of THCV
THC and THCA removal with a quick cycle time
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