Particle Characterisation Particle Size Analyser for Small Particles and Biopharmaceuticals


Shimadzu presented its SALD-7500nano particle size analyser to the professional community during the analytica show. This powerful new tool is ideal for research and development in nanotechnology and life sciences. As the successor of the SALD-7101, the SALD-7500nano is the latest product in Shimadzu’s particle size measurement series. It has been developed for highly sensitive and accurate measurement in the particle size range of nano to sub-micron regions. The system configuration can be optimised to address various applications, purposes, measurement objects, environments and conditions.


The SALD-7500nano is aimed at the following target markets: evaluation of nanoparticles and nanomaterials – the instrumement is able to determine particle size distributions from 7 nm to 800 µm in concentrations from the sub ppm range to 20%; evaluation of aggregation properties in biopharmaceuticals - Aggregate Sizer, a specialised version of the instrument, evaluates aggregation properties of biopharmaceuticals. The Aggregate Sizer measures aggregates in the size range from 7 nm to 800 µm. Furthermore, aggregate concentrations in the sub-visible particle range (from 100 nm to 10 µm) can be evaluated quantitatively (in terms of µg/mL).


The SALD-7500nano covers the measurement range from 7 nm to 800 µm in a concentration range from 0.1 ppm to 20%. Sample amount required is just 5 mL (15 µL with accessories). The continuous measurement function allows real time monitoring while saving measurement data at 1s intervals.


Outstanding functions and features of the SALD-7500nano include built-in self-diagnostic functions for easy maintenance and the measurement assistant functions for preparation of SOPs (Standard Operation Procedures) to ensure that measurements are always performed using the same conditions and procedures. Automatic refractive index calculation function eliminates errors and problems in selection of refractive indices. The laser diffraction method complies with ISO 13320 and JIS Z 8825-1 and enables verification of measurement result validity by referencing light intensity distribution data.


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New Software Speeds Method Development for Laser Diffraction Particle Size Analyser


New software for the Mastersizer 3000 laser diffraction particle size analyser from Malvern Instruments continues to lighten the analytical workload associated with developing robust particle sizing methods for industrial applications. Smart new operational features, such as an Optical Property Optimizer, simplify and streamline the process of method development, while a new result emulation tool eases the process of transferring methods from other particle sizing techniques. A growing range of integrated visual guides, covering


every aspect of the operation of the Mastersizer system, provide the reassurance of Malvern expertise at the bench top.


Laser diffraction systems offer highly automated, easy-to-use, ‘push-button’ particle sizing for diverse applications across industry. However, reaching this level of analytical efficiency requires careful development of a suitable measurement method. The new Mastersizer 3000 v3.00 software simplifies this by aiding users in selecting optical properties. The new version software also supports method transfer, helping laboratories to reach new levels of productivity.


The new Optical Property Optimizer (OPO) allows users to easily manipulate the optical properties necessary to calculate particle size distributions from laser diffraction data, helping them to experimentally determine the ideal settings for a given application. Use of the OPO is explained in an embedded video, which joins the growing series of walkthrough tutorials covering all aspects of laser diffraction analysis for both wet and dry dispersion measurements. Finally, efficiency of a lab can be further enhanced through the application of a new emulation tool that eases method transfer between alternative sizing techniques, such as sieving and laser diffraction.


The Mastersizer 3000 laser diffraction particle size analyser delivers precise, robust measurement of dry powders and liquid dispersions across the milli-, micro- and nano-meter size range, with an extended dynamic range that spans 0.01 to 3500 microns. The new software advances the capabilities of the laser diffraction user interface, supplying those with limited experience of particle sizing with the support needed to confidently access the full capabilities of the Mastersizer system.


30495pr@reply-direct.com New Instrument Measures Cells and Particles as Small as 0.2 Microns


The latest Multisizer 4e from Beckman Coulter Life Sciences features a new 10-micron aperture that enables users to obtain accurate count, size and mass distribution for particles and cells ranging from 0.2 to 1,600 microns. Digital Pulse Processing provides high-resolution analysis in as many as 400 channels for unparalleled performance in pharmaceutical quality control, bioprocessing and industrial applications.


Incorporating the latest technology and software advances, the Multisizer 4e with unique Digital Pulse Processing delivers dynamic size measurements in real time. High-speed signal digitalisation allows the use of various pulse parameters for more accurate cell and particle characterisation. Response is unaffected by particle colour, shape, composition or refractive index. Biotech applications include monitoring of transfection, counting mitochondria, organelles and other intracellular components, and characterising changes in cell size when changes are made to cultures. In these applications the volumetric analysis of the Multisizer 4e provides a more accurate and informative measurement than measuring size alone.


“Counting sub-visible particles, measuring volume as well as count and size, monitoring hundreds of channels at once - all of these features provide analysts with a highly useful and versatile instrument,” said Matthew Rhyner, PhD, Particle Characterisation Senior Global Business Manager at Beckman Coulter Life Sciences.


Sub-visible particle capability means that pharmaceuticals can be analysed for compliance with USP 788 guidelines, to ensure product cleanliness. Multisizer 4e is also compliant with 21 CFR Part 11. Industrial applications range from ink toners to cosmetics, from environmental studies to the monitoring of filter efficiency, and many others.


Instrument operation is completely computer controlled, and intuitive software with wizards and menu driven set-up assists both beginners and experienced users. Sample management system using Smart Technology provides consistency from one analysis to the next.


The Multisizer 4e is the newest member of the COULTER COUNTER product family and the latest advancement in a long line of cell counting and sizing instruments.


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Researchers within the Département Environnement et Agro- biotechnologies (EVA), of the Centre de Recherche Public - Gabriel Lippmann, Luxembourg are using the technique of Nanoparticle Tracking Analysis (NTA) for the characterisation of nanoparticles within their toxicological studies. A team led by toxicologist Dr Arno Gutleb is working closely with colleagues in the Département Science et Analyse des Matériaux (SAM) both to characterise the particles that they produce and to investigate the environmental and toxicological impact of these and other nanomaterials.


For the past 18 months, Dr Gutleb’s team has used the NanoSight NS500, which is now part of Malvern Instruments’ nanoparticle characterisation portfolio, to support the generation and interpretation of environmental risk and toxicity data from nanoparticle testing. With applications ranging from the particle analysis of micelles in foodstuffs to the study of nanosized sediments in water, the NanoSight NS500 has become a highly valued analytical workhorse for a variety of high end research areas.


“The behaviour of nanoparticles varies significantly depending on the medium in which they are found,” explained Dr Gutleb. “Such is the sensitivity of nanosized particles that even seemingly identical media, such as water taken from two different taps, can result in substantial variations in their behaviour. With NTA we are able to sensitively monitor the behaviour of a nanoparticle over time in a variety of different media. This makes it possible to accurately determine the level of environmental risk posed by nanoparticles, allowing us to produce and publish toxicity reports to increasingly higher standards of reliability.”


Over the past year and a half, NTA has been routinely employed by the EVA department for a range of in vitro applications, allowing operators to perform fundamental examinations of nanoparticle behaviour and interactions within a variety of real life media prior to and throughout toxicity testing. The technique provides information on a range of physical nanoparticle characteristics. Its use within EVA is truly varied and researchers perform nanoparticle toxicological testing for a broad range of emerging nanomaterials in the environment, medicine, consumables and geology sectors.


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EVA Researchers use Nanoparticle Tracking Analysis for Toxicology Studies


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