13
Small Volume DNA Quantification
Scientists from Affymetrix, Inc have compared the performance of Tecan’s Infinite 200 NanoQuant multimode microplate reader with other instruments on the market for low volume DNA concentration analysis.
Dr Jim Collins, Director of Assay Development, explained: “We have recently developed a new generation of general cytogenetic assay, requiring precise spectrophotometric quantification of small volumes of DNA. I have always been highly impressed with the quality of instrumentation from Tecan in terms of its robustness and reliability, and the Infinite 200 NanoQuant offers higher throughput than anything else available for quantification of low volumes, making it highly suitable for this assay.”
Vicky Huynh, Jim’s colleague who carried out the evaluation, explained: “The Infinite 200 NanoQuant is a fantastic addition to our laboratory. It is quick, and the software is very easy to use. The NanoQuant Plate™ is compatible with multichannel pipettes for high throughput, and quantification of whole microplates only takes a few minutes. The easy-to-clean plate helps to eliminate cross-contamination and there is no need to dilute samples. It is ideal for both high and low throughput, processing batches of sixteen samples in just one minute with high reproducibility.”
Circle no. 542
Break the Contamination Chain
With swine flu rapidly sweeping across the globe, it is estimated that a third of the world’s population will
be affected by the H1N1 virus. Like seasonal flu, it is believed a second wave of the virus will occur as the virus thrives in winter conditions. The spread of swine flu can be contained if businesses are prepared for the second wave of the virus by providing the correct protective clothing and equipment for employees that will help to break the contamination chain.
Guidance from authorities across the globe is the same; follow good hygiene rules by washing your hands with soap regularly, protecting your nose and mouth when sneezing or coughing, disposing of used tissues promptly and carefully and cleaning hard surfaces, such as door handles, frequently.
In some occupations, it may be necessary to wear protective clothing to help protect against the spread of the swine flu virus. In highly exposed environments, masks and coveralls have a complementary role to play in limiting the spread of the virus. Masks are helpful for infected people to avoid spreading infected droplets to health care personnel, The coverall, used in conjunction with other personal protective equipment such as gloves and disposable respirators, protect professionals from infected droplets and limit the risk of professionals transporting the droplets and the virus to another person.
To ensure effective protection, a protective garment should: be made of a fabric which is a barrier to the virus; designed to avoid cross contamination; have closure and seams which are tight during exposures and after exposure; and be disposed of in a bio-safe way.
DuPont’s range of Tyvek® Classic and Tyvek®
suits, that includes Tyvek® Classic Plus, provides adequate
protection against contamination. From their product portfolio, Tyvek®
Classic Plus (Type 4,5,6) provides a
more adequate barrier by the combination of the fabric, the tight seams and the tight closure of the zipper flap. Tyvek®
Classic (Type 5,6) may provide an adequate
barrier, however, the seams and the zipper coverage are less particle tight than those of Tyvek®
Classic Plus
garments. Both garments are made of the same Tyvek® material which has shown an adequate barrier against infected particles (EN 14126). As a result of its manufacturing process, Tyvek®
has an inherent barrier
that delivers excellent protection and can help against the spread of the swine flu virus.
Circle no. 543
Porvair Sciences has produced a new video that describes and demonstrates the design innovations incorporated into their new Universal Robotic Manifold that enable productive automation of SPE and DNA clean-up procedures.
Accessed from
www.microplates.com/
downloadintro.php the online streaming video shows how the Universal Robotic Manifold offers the unique capability of being able to operate with all brands of filter plate and waste collection reservoir tray. Supplied with an integral vacuum gauge – the Universal Robotic Manifold provides precise and complete control of vacuum pressure ensuring plates may be processed with high reproducibility. The Universal Robotic Manifold is fully compatible with most commercial robotic liquid handling systems.
Visit
www.microplates.com/
downloadintro.php now to see how to accelerate your SPE and DNA clean-up procedures.
Circle no. 544
ADVERTORIAL
Video Demonstrates Automation Friendly SPE Manifold
Just because it’s white doesn’t mean
it’s whiter than white
A rose by any other name should be treated with equal caution – a point that certainly applies to microplates.
Excellent Column-to-Column Consistency Enhances IgG Antibody Purification
Porvair Sciences has announced a new range of Protein A columns that uniquely combine the proven operational benefits of BioVyon technology with the economy and convenience of a cartridge-based separation methodology.
Designed for laboratories looking to handle small numbers of discrete samples - the new mini and micro BioVyon™
Protein A columns offer a simple and rapid method for antibody purification. BioVyon Protein A columns are proven to give consistent recoveries of purified IgG. The high purity and recoveries are consistent in multiple extractions using the same column or from column to column. Each mini column has a capacity of approximately 2.5mg IgG (micro - 200µg IgG) and can be reused several times with appropriate washing steps before the performance degrades. The unique BioVyon solid state media means that performance is not diminished at flow rates as high as 3ml / minute, resulting in faster purifications.
For small-scale preparations, such as screening for IgG expression across a series of cell lines, in which sample is limited and high recoveries are needed the consistency of BioVyon Protein A mini and micro Columns provide unmatched performance.
Circle no. 545
The thorny issue is that the production of polypropylene microplates can involve the use of contaminants, such as plasticizers or mould release agents, which may subsequently affect the accuracy of assay results. This is especially so if you use powerful solvents, such as Ethyl Acetate or DMSO.
Microplates from Porvair Sciences are manufactured at the very edge of injection moulding technology in order to avoid the use of potentially contaminating chemicals. Call, email or log on to
www.porvair-sciences.com/
downloadintro.php
to request a copy of an independent article, together with a sample microplate, so you can compare results with your current brand.
Then you’ll see who’s whiter than white.
Porvair Sciences Ltd Unit 6 Shepperton
Telephone +44 (0) 1932 240255
int.sales
297 Kingston Rd, Leatherhead, Surrey KT22 7PL, UK. Tel: 01372 824290 • Fax: 01372 824271 E-mail:
int.sales@porvair-sciences
www.porvair-sciences.com
Porvair Sciences Ltd, Dorset House, Regent Park Govett Avenue
UK
Email: @
porvair-sciences.com
www.porvair-sciences.com
Circle no. 546
Shepperton Business Park Middlesex
TW17 8BA Fax +44 (0) 1932 254393
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