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Volume 24 Number 6 January 2019


table of contents


Dear Abbe


Dear Abbe, Last week, one of my students returned to the lab to fin-


ish up an experiment and claims as she viewed her cultured cells under the microscope that they kept telling her to “use the force!” She feels that the cells were telling her to expand her studies to include atomic force microscopy, while I think they were urging her to get on with the already planned experi- ments with optical tweezers. As someone knowledgeable about all things small, what do you think is the correct interpretation of her cells’ message? Perplexed in Tennessee


Microscopic Chemical Characterization and Reactivity in Cementing Systems of Elephant Grass Leaf Ashes Josefa Roselló, Lourdes Soriano, Holmer Savastano Jr, M. Victoria Borrachero, Pilar Santamarina and Jordi Payá


Secondary fluorescence in WDS: the role of spectrometer positioning Ben Buse, Jon Wade, Xavier Llovet, Stuart Kearns and John J. Donovan


Multi-Angle Plasma Focused Ion Beam (FIB) Curtaining Artifact Correction Using a Fourier-Based Linear Optimization Model Christopher W. Schankula, Christopher K. Anand and Nabil D. Bassim


Ultrastructural Deformities in the Scales of Cyprinus carpio Inhabiting Two Government- Sponsored Fish Farms in North-East India Contaminated by Municipal Wastes and Other Pollutants Eva M. Pala


Functional Imaging of Neurotransmitters in Hymenolepis diminuta Treated with Senna Plant Trough Light and Confocal Microscopy Bidisha Ukil, Suman Kundu and Larisha Mawkhlieng Lyndem


Tree-Dimensional Reconstruction of Erythrocytes Using the Novel Method For Corrective Realignment of the Transmission Electron Microscopy Cross-Section Images Yuzhou Fan, Djordje Antonijević, Xing Zhong, Vladimir S. Komlev, Zhiyu Li, Marija Đurić and Yifang Fan


Morphological Properties of the Two Types of Caudate Interneurons: Kohonen Self- Organizing Maps and Correlation-Comparison Analysis Ivan Grbatinić, Bojana Krstonošić, Dušica Marić and Nebojša Milošević


Microstructural, Mineralogical and Petrographical Characteristics of the Medieval Ceramics from the Studenica Monastery (Unesco World Heritage Site): Implications on the Pottery Technology and Provenance of Te Raw Material Kristina Šarić, Vesna Bikić and Suzana Erić


Electron Beam-Induced Carbon Erosion and the Impact on Electron Probe Microanalysis Mike B. Matthews, Stuart L. Kearns and Ben Buse


Effect of Heavy Metals Contamination from Cigarette Smoke on Sound and Caries-Like Enamel Jéssica D. Teobaldo, Waldemir F. Vieira-Junior, Anderson Catelan, Maria do Carmo A. Mainardi, Orlando A. Ysnaga, Ubirajara P. Rodrigues-Filho, Giselle Maria Marchi, Débora A. Lima and Flávio H. B. Aguiar


Weka Trainable Segmentation Plugin in ImageJ: A Semi-Automatic Tool Applied to Crystal Size Distributions of Microlites in Volcanic Rocks Charline Lormand, Georg F. Zellmer, Károly Németh, Geoff Kilgour, Stuart Mead, Alan S. Palmer, Naoya Sakamoto, Hisayoshi Yurimoto and Anja Moebis


Compressed Sensing of Scanning Transmission Electron Microscopy (STEM) With Nonrectangular Scans Xin Li, Ondrej Dyck, Sergei V. Kalinin, and Stephen Jesse


A New and Unexpected Spatial Relationship Between Interaction Volume and Diffraction Pattern in Electron Microscopy in Transmission Etienne Brodu, and Emmanuel Bouzy


Te Impact of Two Combined Oral Contraceptives Containing Ethinyl Estradiol and Drospirenone on Whole Blood Clot Viscoelasticity and the Biophysical and Biochemical Characteristics of Erythrocytes Odette Emmerson, Janette Bester, Barend G. Lindeque and Albe C. Swanepoel


Exploring Photothermal Pathways via in Situ Laser Heating in the Transmission Electron Microscope: Recrystallization, Grain Growth, Phase Separation, and Dewetting in Ag0.5Ni0.5 Tin Films Yueying Wu, Chenze Liu, Tomas M. Moore, Gregory A. Magel, David A. Garfinkel, Jon P. Camden, Michael G. Stanford, Gerd Duscher and Philip D. Rack


Regulation of Vascular Smooth Muscle Cell Stiffness and Adhesion by [Ca2 + ]i: An Atomic Force Microscopy-Based Study Yi Zhu, Li He, Jing Qu and Yong Zhou


2019 January • www.microscopy-today.com doi:10.1017/S1551929518001207 49


Dear Perp, First of all, if you are trying to imply something about my


being “knowledgeable about all things small,” let me ASSURE you that I have NO problems in that area. But as to your ques- tion, it is possible that they were referring to either AFM or optical tweezers. It is possible that she simply misheard them. If these were CHO cells, they can have a very difficult accent to comprehend, and if they were HEK 293 cells, well then all bets are off since these snarky cells are well known to be lazy roustabouts who simply like to stir up trouble. Is it possible that they said, “Use the horse,” in trying to get her to switch to E. Derm (NBL-6), an equine dermal fibroblast line, or even “Use the fourths,” in an effort to improve a musical composition? While unlikely, they may even have been trying to steer her towards the research of my dear friend John “Pinkie” Schwartz of the Des Moines Still College of Osteopathy. Pinkie was great at culturing cells. He not only got a line of endothelial cells to appreciate Wagner, but as a party trick they would, on com- mand, shout “May the Schwartz be with you.” Cracked me up every time! I myself have only heard cells speak outloud on a very few occasions, and I usually ended up waking in a strange hotel room with a splitting headache aſterwards. If it were me, I would autoclave the snippy little jerks. If you hear your cells speaking to you, you need Herr Dr. Abbe’s


advice more than most researchers. Make a recording and send it to Dr. Abbe’s faithful clinical assistant at jpshield@uga.edu.


Volume 26 Number 5


2018 September


www.microscopy-today.com


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