Microbial-Induced Calcite Precipitation


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Figure 6: Inactive bacteria (a) on the surface of the fracture surface and (b) enclosed within the precipitation product.


surface of sample. Sand particles were uniformly covered by CaCO3


show calcium carbonate (CaCO3) formation on the fracture layers aſter four treatments, allowing sand grains to


bond with each other. Te milling function of the FIB produced a cross section revealing the interior structure of the carbon- ate-sand bonding. Te ability to image and analyze μm-scale structures of the bio-mediated soil materials was essential for the study of this bio-geo material.


Acknowledgements Tis article is based on work supported by the National Science Foundation Grant No. 1531382.


References [1] JT DeJong et al., J Geotechn Geoenviron 8 (2011) 1–15. [2] BC Martinez et al., J Geotechn Geoenviron 139 (2013) 587–98. [3] M Burbank et al., J Geotechn Geoenviron 139 (2013) 928–36. [4] AA Qabany et al., J Geotechn Geoenviron 138 (2012) 992– 1001.


[5] Q Zhao et al., J Mater Civil Eng 26 (2014) 04014094. [6] M Dyer et al., Bio-inspired, Biomimetic and Nanobiomate- rials 6 (2017) 168–83.


[7] C Chou et al., J Geotechn Geoenviron 137 (2011) 1179–89. [8] MA Groeber et al., Mater Charact 57 (2006) 259–73.


2019 January • www.microscopy-today.com Wavelength (nm)


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