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MicroscopyEducation


Figure 2: Detection of stomata for density measurements on a leaf of the Blue Glow Agave plant. (a) Specimen prepared by leaf peeling, and (b) varnish replica of leaf surface showing stronger image contrast on a similar field of view. The leaf axis is vertical in these pictures.


role and behavior of stomata provides guidance that can lead to successful foliar fertilization [6]. Plant diseases. Some pathogens can modulate stomatal


behavior, causing the stomata to open so that the pathogens can enter the plant. For example, Salmonella enterica stimu- late lettuce guard cells and penetrate through their stomata. Research directed toward understanding pathogen modula- tion of stomatal defenses could lead to new strategies for the prevention of plant diseases [7]. Fires. Planting selected species of trees could help reduce


wildfire risk. For example, the arrangement of stomata in the leaves of the Mediterranean cypress allows this tree to retain a high water content even in conditions of high heat and drought, possibly reducing fire risk [8]. Tis article shows how stomata can be studied in detail with inexpensive equipment


found in many high school 2019 January • www.microscopy-today.com


Figure 3: Agave “Blue Glow” hybrid monocot plant image (top) followed by planar and cross-sectional views. The bottom image shows a cross-sectional view of two stomata, indicated by arrows. The leaf axis is vertical in the center image and perpendicular to the page in the bottom image.


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