Quantifying Polymer Deposition
Table 2: Results from microfluorometry of 20 hair fibers treated for 3min with solutions of 0.1%, 0.01%, and 0.001% polymer. Average values are shown at the bottom: mean (μ), standard deviation (σ), and standard error (σE
). Fiber
Figure 6: Distance scans across the exterior of hair fibers treated with: 0.1% polymer solution (dark blue), 0.01% solution (red), 0.001% solution (green), and control without polymer solution (black).
Results Microfluorometry of the cuticle. Te amount of polymer
deposited on the hair fibers was determined by monitoring the FI obtained from surface scans of the cuticular surface of the hair fibers. To test the analysis system, FI was measured at 800 points along the length of a single hair fiber from each treatment as well as from the control to determine the poly- mer deposition profile along a 3 mm length of the hair fiber (Figure 6). To obtain statistically useful values for the amount of
polymer covering the surface of the hair fibers, FI measure- ments were acquired from each of 20 different hair fibers for each treatment. Table 2 shows results of these microfluorom- etry measurements. Te mean (μ) values of the fluorescent intensities 54.4>23.4>12.0 followed the concentration differ- ences of the polymer solutions: 0.1% > 0.01% > 0.001%. Epi-fluorescent images. To obtain an epi-fluorescent image,
the illuminator directed excitation light onto the specimen by first passing the light through the microscope objec- tive lens that acts as a condenser on the way toward the specimen. Ten the same objective captures the emitted fluorescence. Figure 7 shows typical epi-fluorescent light
micrographs obtained from the treated and con- trol hair fibers. Intensities of the epi-fluorescent images obtained from the hair fibers were consis- tent with the distance scans of Figure 6 and the data of Table 2: the hair fibers treated with the 0.1% polymer solution had the highest FI while those with the 0.001% solution had the lowest. Te FI of the control was barely above background level. Microfluorometry of the cross sections.
Previous microscopy (not shown) of the pathway for diffusion of polymer into hair fibers indicates that molecules enter the hair fibers at the edges between the cuticle cells. At the beginning of the diffusion process intercellular diffusion is the preferred route, predominantly along the non- keratinous regions of the cell membrane complex
28
# 1 2 3 4 5 6 7 8 9
10 11 12 13 14 15 16 17 18 19 20 µ σ
σE 0.1%
Solution FI
89.63
106.48 55.18 21.33 35.94 99.83 27.7 18.5
26.89 51.05 80.04 73.21 55.77 61.21 35.13 71.08 25.75 27.34 33.09 93.24 54.42 28.51 6.38
0.01% Solution
FI
94.32 49.19 18.68 12.73 21.99 19.43 21.24 6.43
21.82 16.23 27.91 2.97
24.53 20.42 28.14 27.65 10.95 3.77
18.44 22.87 23.49 19.53 4.37
0.001% Solution
FI 5.69
13.96 18.12 4.14 7.32
18.79 10.78 16.85 3.94
18.41 28.56 6.22
17.50 8.27
11.35 10.56 9.54 6.23
10.07 12.74 11.95 6.24 1.40
Control FI
2.09 7.3
3.52 1.78 3.51 2.27 3.05 3.04 1.08 2.72 4
2.02 0.6
3.04 1.79 3.95 0.66 7.12 2.66 3.79 3.00 1.76 0.39
Figure 7: Epi-fluorescent light microscopy images from hair fibers treated with: (A) 0.1%, (B) 0.01%, (C) 0.001% polymer solution, and (D) control (no polymer solution). Image widths=1.4mm.
www.microscopy-today.com • 2019 July
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