SCIENCE REVIEW
‘The study of KIR genes has enabled researchers to establish an association between their presence or absence and the occurrence of viral and bacterial infection and autoimmune diseases’
et al. Genotyping and follow-up of HR-HPV types detected by self-sampling in women from low socioeconomic groups not participating in regular cervical cancer screening in France. J Clin Virol 2016; 78:102–7). A cytological and/or histological follow-up was undertaken for a subset of women harbouring HR-HPV types. An Abbott real-time high-risk HPV test was used for screening, while the INNO-LiPA HPV Genotyping Extra assay was used for genotyping. A total of 4245 self-samplings were
performed, out of which 609 (14.3%) were high-risk HPV (hrHPV)-positive by the screening test, including 114 HPV16 (18.7%), 41 HPV18 (6.7%), and 454 hrHPVnon-16/18 (75.4%). A sample of 260 out of the 454 hrHPVnon-16/18 were genotyped, revealing HPV52 (35%), HPV66 (22.6%), HPV51 (19.6%), HPV31 (15.7%), HPV39 (13%), HPV56 (10.4%) and HPV53, -35, -59, -33, -58, -82, -45, -68 and -73 (<10% each). After 12 months a second self-collection
kit was sent to 274 of the 609 hrHPV-positive women who had not had a previous smear
test. Of these women, 130 provided a sample for HPV testing; one was uninterpretable, 56 were HPV-negative and 73 were hrHPV- positive (10 HPV16, three HPV18, 60 hrHPVnon-16/18). Of the 345 women with cytological and/or histological follow-up, 19 (5.5%) had ≥CIN2 lesions (11 were HPV16- positive and eight were hrHPV non-16/18- positive). The authors believe that this illustrates the
potential efficacy of self-sampling as a cancer screening strategy for socioeconomically deprived women who do not participate in regular screening programmes. Finally, a group working in Cairo (Youssef
MA, Abdelsalam L, Harfoush RA et al. Prevalence of human papillomavirus [HPV] and its genotypes in cervical specimens of Egyptian women by linear array HPV genotyping test. Infect Agent Cancer 2016; 11: 6) has investigated HPV genotype distribution and co-infection occurrence in cervical specimens from Egyptian women. A group of 152 women with and without
cervical lesions was studied, all of whom had cervical cytology and HPV testing. They were classified according to cytology into those
with normal cytology, with squamous intraepithelial lesions (SIL) and invasive squamous cell carcinoma (SCC). Cervical samples were analysed to identify the presence of HPV by PCR, and all positive HPV-DNA samples underwent viral genotype analysis by LA HPV genotyping assay. A total of 26 HPV types with a prevalence
of 40.8% were detected. This prevalence was distributed as follows: 17.7% among cytologically normal females, 56.5, 3.2 and 22.6% among those with LSIL, HSIL and invasive SCC, respectively. Low-risk HPV types were detected in 81.8% of the cytologically normal women, in 5.7% of those with LSIL and in 14.3% of those with invasive SCC, while no low-risk types were detected in HSIL. High-risk HPV types were detected in 18.2% of infections in the cytologically normal women, 14.3 % of infections in LSIL, and in 21.4% of invasive lesions. The probable and possible carcinogenic HPV types were not detected as single infections. Mixed infection was present in 80% of women with LSIL, in 100% of those with HSIL and in 64.3% of those with invasive SCC. The Egyptian authors believe that these
data expand the knowledge concerning HPV prevalence and type distribution in Egypt, and may help to create a national HPV prevention programme.
A Science Review compiled by the Editor.
Connecting Global Competence F3 U2 TU RE4 NE3 TW O4 Elementary to your success.
The world’s largest network of trade fairs for laboratory technology, instru- mental analysis and biotechnology features the complete range of products and services for all of your laboratory needs—in industry and research. The scientifi c highlight is the analytica conference, where the international elite discusses analysis trends in chemistry and the life sciences.
Contact: Pattern Ltd., Tel. +44 20 3375 8230,
info@pattern.co.uk
May 10–13, 2016 Messe München
25th International Trade Fair for Laboratory Technology, Analysis, Biotechnology and analytica conference
www.analytica.de
Highlights in 2016:
Live Labs and occupational safety
RK GL 03 B2 AL2
THE BIOMEDICAL SCIENTIST MAY 2016
235
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60