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PRODUCT FOCUS TIME-RESOLVED SPECTROSCOPY


Microscopy Lightsheet Z.1


Zeiss has developed a light sheet microscope for 3D observations of biological samples over very long periods of time. The microscope incorporates a MEMS scanner developed by the Fraunhofer Institute for Photonic Microsystems IPMS. The light sheet fluorescence microscope


system, Lightsheet Z.1, gives researchers the opportunity to observe dynamic processes in large living organisms. Compared to former methods of fluorescence microscopy such as confocal microscopy, this system is characterised by a lower light load on the specimens, opening up new possibilities for gentle, long-term examinations of living organisms in 3D. Light sheet fluorescence microscopy (LSFM) allows only the relevant volume and not the entire specimen to be illuminated for a section of the fluorescence-marked tissue by a very thin, expanded laser beam, the so-called light sheet. www.ipms.fraunhofer.de/en.html


FluoView multiphoton microscope system


Olympus has released its FluoView FVMPE-RS, a dedicated multiphoton microscope system. With its high speed and precision performance, the FVMPE-RS is designed for electrophysiology and optogenetics studies. It is also a good match for applications such as high-speed calcium and in vivo imaging, peristalsis and blood flow studies, mosaic imaging, connectomics and functional brain imaging and stem cell research. www.microscopy.olympus.eu


A question of time


The latest releases of instruments used to study the chemical constituents of dynamic processes


The IsoPlane SCT-320 spectrograph from Princeton Instruments features a new optical design that eliminates the primary aberrations present in traditional Czerny- Turner imaging spectrographs. IsoPlane means researchers can use the full spatial extent of the detector without loss of spectral or spatial resolution. It focuses data sharply at every wavelength and allows the unrestricted use of large- format detectors, and directs photons to where they are supposed to go. With the IsoPlane


Sensors and detectors


Photodiode for electron detection Opto Diode has introduced the 20mm2


photodiode


with a circular active area for electron detection. The device offers 100 per cent internal quantum efficiency and high stability. The absence of a surface dead region results in 100 per cent collection efficiency. The radiation-hard, junction-passivating, oxynitride protective entrance window makes the device stable even after exposure to intense flux of UV photons (showing less than 2 per cent responsivity degradation after MJ/cm2


of


254nm and tens of kJ/cm2 of 193nm photon exposure). The radiation-hardness makes these diodes suitable for space missions and satellite applications. The product features a typical rise time of 1.7µsec, a minimum rise time of 0.8µsec, and a maximum of 3.3µsec. Housed in a TO-8 package, the UVG20C offers peak responsivity of 800nm at approximately 0.56 A/W. www.optodiode.com


www.electrooptics.com | @electrooptics


spectrograph more photons end up in the peak, increasing the height and effective signal-to-noise ratio (SNR), rather than in the wings where they contribute to the background noise. Applications for the IsoPlane spectrograph include: time-resolved spectroscopy; multi- channel spectroscopy; micro-spectroscopy, including Raman spectroscopy, fluorescence and photoluminescence; laser-induced breakdown spectroscopy (LIBS) and similar high-resolution techniques; Fourier-domain spectroscopy; and biomedical imaging. www.princetoninstruments.com


Ultrafast Systems, headquartered at Sarasota Florida, provides a wide


range of spectrometers for optical absorption and emission investigations. Its Helios absorption spectrometer offers broad spectral coverage from the UV to the NIR, with an operational time window of up to 8ns. It has an intrinsic resolution of 7fs. Helios-IR is an option that extends the wavelength coverage into the mid-infrared range, while Eos is a version of Helios with a time window that extends through the nanosecond


which includes Fluorescence Lifetime Imaging (FLIM) and Time-resolved Luminescence Microscopy (TLM). This combination facilitates the acquisition of additional material contrasts hidden in the time function of a fluorescence or luminescence signal and allows them to be perfectly linked with Raman, SNOM or AFM imaging. The technology enables a variety of measurement possibilities for an improved and more comprehensive understanding of a sample’s properties. StrobeLock is comprised of a pulsed excitation laser combined with a Time-Correlated Single-Photon Counting (TCSPC) detector. The ability to switch between time- resolved and conventional


and microsecond regions, employing a photonic crystal fibre for probe light generation.


Halcyone is a fluorescence measurement system comprising a Fluorescence Up-conversion spectrometer and a Time-Correlated Single Photon Counting (TCSPC) instrument all in one box. In the up-conversion mode Halcyone has a time window of several nanoseconds and femtosecond time- resolution. In the TCSPC regime the time window extends to microseconds and beyond with around 200ps resolution. www.ultrafastsystems.com


Based in Ulm, Germany, WITec’s Alpha300 and Alpha500 microscope series can be integrated with StrobeLock imaging modes,


mode allows the microscope user to choose the preferred measurement technique. The StrobeLock electronics are integrated into WITec’s control unit, AlphaControl, meaning time-correlated measurements can be linked. Detectors and excitation lasers are adjusted to the WITec microscope series for imaging sensitivity and ease- of-use. www.witec.de


NOVEMBER 2013 l ELECTRO OPTICS 35


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