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Environmental and food safety standards are rising. Wherever higher sensitivity is required but sample material is limited or analyte enrichment steps are impractical, capillary flow LC-MS will become a useful alternative. In clinical proteomics research there is a push towards higher throughput while good sensitivity has to be maintained. Therefore, shotgun profiling of clinical samples will benefit greatly from this technology. It can be envisioned that research driven proteomics will generate marker signatures on large patient cohorts. In a second step the identified markers could be used for an individualised patient diagnosis.
In conclusion, capillary flow LC-MS is becoming a useful alternative for highly sensitive and selective analysis of complex samples. In bioanalysis it will continue to penetrate different application areas. It has already shown a lot of promise and more and more laboratories are adopting capillary LC-MS for routine applications. Still, further proof is required to convince more conservative LC-MS users that this method is mature enough to be broadly used. However, the first steps are made and we are
confident that further evidence and proof of the true capabilities of this technique will be generated in the next years.
References
1. Dong MW. Myths in Ultrahigh-Pressure Liquid Chromatography. LCGC NA. (2013) 31(10):868-880
2. Köcher T, Pichler P, De Pra M, Rieux L, Swart R, Mechtler K. Development and performance evaluation of an ultralow flow nanoliquid chromatography-tandem mass spectrometry set-up. Proteomics. (2014) 14(17-18):1999-2007
3. Scheltema RA, Hauschild JP, Lange O, Hornburg D, Denisov E, Damoc E, Kuehn A, Makarov A, Mann M. The Q Exactive HF, a Benchtop Mass Spectrometer with a Pre-filter, High-performance Quadrupole and an Ultra-high-field Orbitrap Analyzer. Mol Cell Proteomics. (2014) 13(12): 3698–3708
4. Arnold DW and Needham SR. Micro- LC-MS/MS: the future of bioanalysis. Bioanalysis (2013) 5(11):1329-1331
5. Chappell DL, Lassmann ME, McAvoy T, Lin M, Spellmann DS, Laterza OF. Quantitation of human peptides and proteins via MS: review of analytically validated assays. Bioanalysis (2014) 6(13):1843-1857
6. Vissers JPC, Claessens HA, Cramers CA. Microcolumn liquid chromatography: instrumentation, detection and applications. J Chrom A. (1997) 779:1-2
7. Wilm M and Mann M. Analytical Properties of the Nanoelectrospray Source. Anal Chem. (1996) 68(1):1-8
8. Baker M. Blame it on the Antibodies. Nature. (2015) 521:274-276
9. Bradbury A and Plückthun A. Standardize antibodies used in research. Nature. (2015) 518:27-29
10. Van den Broek I and van Dongen WD. LC-MS-based quantification of intact proteins: perspective for clinical and bioanalytical applications. Bioanalysis. (2015) 7(15):1943-1958
New GC Column Line
Shimadzu, is expanding its product portfolio of accessories and consumables by introducing a series of high-quality GC capillary columns. In this way, Shimadzu perfects the objective of providing full solutions to its customers.
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