Coming Events 2015
MSC-SMC Annual Meeting May 26–29, 2015
Hamilton, Ontario, Canada
www.bimr.ca/events/msc-smc-annual-meeting-2015
Inter/Micro 2015 June 8–12, 2015 Chicago, IL
www.mcri.org
SCAMDEM2015 June 9–11, 2015 Jyväskylä, Finland
www.jyu.fi /en/congress/scandem2015
Live-Cell Imaging June 23–25, 2015 Norwich, UK
www.physoc.org/live-cell-imaging-23-25- june-2015
Microscience Microscopy Conference June 29–July 2, 2015 Manchester, United Kingdom
www.mmc2015.org.uk
Stereology & Image Analysis July 6–10, 2015 Liege, Belgium
www.14icsia.com
Semicon West, 2015 July 14–16, 2015 San Francisco, CA
www.semiconwest2015.org
Microscopy & Microanalysis 2015 August 2–6, 2015 Portland, OR
www.microscopy.org 2016
Microscopy & Microanalysis 2016 July 24–28, 2016 Columbus, OH
www.microscopy.org 2017
Microscopy & Microanalysis 2017 July 23–27, 2017 St. Louis, MO
www.microscopy.org 2018
Microscopy & Microanalysis 2018 August 5–9, 2018 Baltimore, MD
www.microscopy.org 2019
Microscopy & Microanalysis 2019 August 4–8, 2019 Portland, OR
www.microscopy.org
More Meetings and Courses Check the complete calendar near the back of this magazine.
T e Nobel Prize in Chemistry was awarded last year for the development of super-resolved fl uorescence microscopy, which is now an established method in the armamentarium of microscopists. However the specialized microscopes for super- resolution remain expensive and complicated to use. Fei Chen, Paul Tillberg, and Edward Boyden have developed an entirely new approach to super-resolution [ 1 ]. Instead of using an instrument that can resolve objects smaller than the diff raction limit described by Ernst Abbe, Chen et al. decided to make the object of study larger so that a diff raction-limited microscope can image a structure that could only be otherwise imaged by electron microscopy or super-resolution microscopy. Chen et al. discovered that by synthesizing a swellable polymer network within a specimen, it can be expanded, resulting in a physical magnifi cation of four- to fi ve-fold. T e other key was the development of specifi c labels that could be applied to the specimen and then covalently anchored to the polymer network. T ese labels (for example, anti-tubulin to label microtubules) originally would be spaced below the diff raction limit, but they are then isotropically separated when the polymer network swells and can be optically resolved with a diff raction-limited microscope. Chen et al. fi rst set out to see whether a well-known property of polyelectrolyte gels—namely that dialyzing them in water causes expansion of the polymer network into extended confi rmations—could be performed in a biological sample. T ey infused sodium acrylate, a monomer used to produce superabsorbent materials, along with other monomers, cross-linkers, and an accelerant into chemically fi xed and permeabi- lized specimens that had been labeled. T e specimen-polymer composite was treated with protease to digest the specimen but leſt the labels bound to the polymer. Dialysis with water then resulted in a 4.5-times linear expansion. Quantitative measurements and statistical analysis determined that the spatial error was less than 1%. T ey also determined that the fl uorescent label targeted to a biomolecule of interest remained
Carmichael’s Concise Review A New Approach to
Super-Resolution Microscopy
Stephen W. Carmichael Mayo Clinic , Rochester , MN 55905
carmichael.stephen@
mayo.edu
Figure 1 : A volume-rendered image from CA1 layer of the mouse hippocampus showing neurons (expressing cytosolic yellow fl uorescent protein in green), presynaptic elements (stained with anti-Bassoon, blue), and postsynaptic elements (stained with anti-Homer1, red). The scale bar in the X direction is 13.5 μ m, Y is 7.3 μ m, and Z is 2.8 μ m.
8 doi: 10.1017/S1551929515000383 2015 May
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