NetNotes
Image Processing: offl ine TIA viewer
How can I view.emi
and.ser fi les on Windows? Is there any offl ine TIA viewer for Windows available? Ravi T akkar
ravi.thakkar369@
gmail.com Fri Jan 2 T is won’t help entirely, but ImageJ has a plugin that can open
the.SER fi les. You need to be sure that you point to the correct fi le that contains the image. Most of the experimental information is stored in the.EMI fi le. T e FEI website has a link to an off -line version of ESVision (
http://www.fei.com/service-support/es-vision/) which is the original version of TIA. You may be able to run it and analyze the data directly. I’ve not tested it. Henk Colijn
colijn.1@osu.edu Fri Jan 2
EM: hardware and software for remote viewing
As part of a pitch to install remote viewing / webinar capabilities in our microscopy lab, my administration as asked me to fi nd example systems and collect information to help us optimize and balance our wants, needs, and costs. If you have an electron microscope that you can remotely view, remotely operate, send the display output to a classroom, send the display outside your institution’s network for a webinar, etc. and are willing to answer some questions about components and costs would you please contact me. Stefanie Brachfelds brachfelds@mail.
montclair.edu T u Feb 26
You can also look at this site. http://telepresencecollaboratory. org, Telepresence Collaboration, which is fairly old. We started doing it at ANL when the Mosaic WWW browser fi rst appeared ~ 1994. T ere are also a few old PDF’s of Lectures here:
http://tpm.amc.anl. gov/Lectures/ Today, there are numerous solutions that update these older protocols. To be honest, because of network safety (i.e. hacker) issues, I no longer allow remote control, only passive observation. I would caution you that you will need to do the same. All publically accessible sites get attacked. You don’t want to know how many hackers try to break into the Microscopy Listserver every day. Nestor Zaluzec
anl.nestor.zaluzec@
gmail.com Fri Feb 27
TEM: preventing charging
Does anybody use a coater to coat ceramic TEM samples with a very thin layer to mitigate charging? I would also be happy to know if there are any other ways to stop charging of samples. I am looking at manually polished complex oxide samples. Debangshu Mukherjee
debangshu@psu.edu Sat Feb 28
We’ve had good luck putting down a few nm (usually 3 nm on our system) of carbon to mitigate charging and to stabilize the Formvar fi lms our bio friends make. If you are doing atomic resolution STEM, make sure the C is on the bottom side. Henk Colijn
colijn.1@osu.edu Sat Feb 28
TEM: holder insertion/extraction problems
We have a custom holder for the JEOL 2100 but have been having problems with insertion and extraction. T e holder does not get “sucked in” by the vacuum aſt er the fi rst and second rotations. It actually requires a little pushing, and sometimes extraction is very diffi cult, requiring more force than a standard holder. T e puzzling thing is that the holder works perfectly in a JEOL 2010F. T e holder manufacturer has rechecked all physical dimensions of the holder and the o-rings, fi nding them within tolerance. JEOL also has taken back the goniometer on repeated occasions but has not found a solution. Oddly, a loaner goniometer JEOL installs when they take ours away, happens to work without problems. Last incident we had, the vacuum
2015 May •
www.microscopy-today.com
was breached upon holder extraction. While pulling the holder, the outer gasket on the goniometer was extracted too. What is the most likely cause for this? We see wear marks on the alignment pin of the holder. Rodrigo Bernal
robernal@u.northwestern.edu Mon Jan 26 It sounds like a goniometer problem. Wear marks on the alignment pin are not good because the inner tube of the goniometer can be scratched. T is tube is a rotating part that moves with the holder and scuffi ng is possible between this tube and the internal wall of the goniometer. Scratches on the inner tube can weaken the vacuum sealing during the movement of the holder. If this hypothesis is correct, it’s necessary to remove this tube to get it into the shape of the goniometer wall (by soſt abrasion with Pikal for example). T is is a job for a JEOL engineer. If there are scratches this inner tube must be replaced. T is is a tricky job and expensive part. Each vacuum leak during holder push/pull movement is bad experience for the ion pump. Nicolas Stephant
nicolas.stephant@
univ-nantes.fr Tue Jan 27
TEM: monitor turned black
When coming back from holidays, I found our CM10’s monitor display was completely dark—A small part of the data display on a corner of the screen has disappeared or faded for a while but the machine was operational without any problem. Now the whole screen has blacked out. T e Data Dim and Panel Dim knobs were usually set at low or off during non-usage times. Other panel lights are all normal. It seems like a small part (bulb or circuit board?) wearing out. Is there any simple way to check or order/replace the part? Guosheng Liu
guosheng.liu@
usask.ca Mon Jan 5 One thing you can try is to shine a bright light up in the upper
leſt corner of the Data Monitor and see if the characters show up. T e Data monitor intensity circuitry is a little strange. When you adjust the Data Dim knob it controls the intensity of a bulb on a circuit board behind the panel. T ere is a light absorbing diode that is next to the bulb that measures the intensity and adjusts the circuitry that controls the Data brightness. If this bulb is burned out, then the monitor goes black. John Schreiber
js51@princeton.edu Mon Jan 5
SEM: vacuum hose source
First, thanks to everyone for all the excellent advice I’ve received in the past on this list. I’ve got the rather unique task of running an SEM program accessible to the general public at a makerspace in Chicago, as a volunteer with no formal training and a shoestring budget. As far as I know, our circumstance is rather unique. T ere’s no way I could have managed this long without all the help I’ve received here. I managed to track down and repair the problem with wildly fl uctuating vacuum readings (an extremely dirty Penning gauge) and the reason the vacuum was really bad aſt er I fi xed it (a leaking air admit solenoid.) But a bad shaſt coupling on our Edwards E2M12 rotary pump was causing it to overheat and burn its oil, and, unfortunately, splattering said burnt oil up the rubber vacuum hose. I secured funding to get the pump overhauled, but now I need to know what to do with the hose. I fi gure it needs to be replaced, seeing as it reeks of burnt oil. What I’m measuring seems to be about 7/8 I.D. and 1/4" wall thickness, so about 1 3/8" O.D. It’s diffi cult to get a good reading as the hose ends were either clamped around something or stretched. Looking at
http://www.pchemlabs.com/subcatagoryb.asp?pid=Pure- Gum-Rubber it looks like the gum rubber vacuum hose they sell jumps from 13/16" I.D. with 3/8" walls (listed as used for KF16) 1" with 1/2" walls (listed as used for KF25). T e pump has a KF25 fi tting, and the hose slips over a copper pipe in a concrete block as a vibration damper. But that size seems like it would be too large. McMaster-Carr is local for us, so I checked
http://www.mcmaster.com/#abrasion-resistant-gum-rubber-tubing/
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