Cellular


Immunology Carolina BioKits®


Investigation


For a class of 30 students, working in teams of 2. Students use the dou- ble diffusion technique in agar gel to study the antigen-antibody reaction. Note: Contains perishable items; please give use date on order. Sold only to schools and businesses.


Kit includes: 15 Petri Dishes 4 Bottles Saline Agar (100 mL each)


Pipets


Vials Green and Red Dyes Vial Barium Chloride Vial Potassium Sulfate Vial Bovine Albumin Vials Goat Anti-Bovine, Anti-Horse, Anti-Swine Albumin


Bottle Saline Plastic Cups Craft Sticks


Understanding Immunology Booklet


Teacher’s Manual with Reproducible Student Guide


202100 Per kit : Immunodetective A B


Center Well


Center Well Swine Albumin


Well A Anti-Bovine Albumin Antigen-Antibody Sets


For demonstrating immunological precipitin reactions. Set includes 5 mL saline (diluent); 1.5 mL whole pooled serum containing the natural concentration of albumin (the test antigen); 1 mL goat antiserum containing antibody specific for albumin; and Understanding Immunology booklet. Each chemical is in a vial with fine-tipped dropper suitable for filling diffusion wells.


202105 Bovine Serum/Anti-Bovine Albumin Each 202106 Horse Serum/Anti-Horse Albumin 202107 Swine Serum/Anti-Swine Albumin


Each Each


Animal Blood Sera Sets


For performing qualitative antigen-antibody precipitation test by double diffusion in agar gel (Ouchterlony analysis). Use in experi- ments of your design or with item #202100 Carolina BioKits®


:


Immunodetective Investigation. Provided in vials with fine-tipped droppers convenient for filling wells. Exercises outlined in our instructions require both items #202101 and #202102 sets. Note: Perishable; please give use date on order.


Serum Antigen Set


Whole pooled sera containing natural concentrations of albumin (the test antigen). Sera can be combined or diluted as desired in the disposable mix- ing cups provided. Bovine Serum (1.5 mL) Vial Saline Solution


Swine Serum (1.5 mL) 12 Microtip Droppers Horse Serum (1.5 mL) 12 Plastic Mixing Cups


3 Droppers, 41 202101 Per set 202118 Meat Adulteration Test Kit


Meat Adulteration Test Kit Employs specific antibody-antigen reactions in agar gel to detect the presence of horse meat and the percentage of that adulteration in fresh ground beef. Indica- tors for the meats are their unique blood serum albumins (the antigens). Double immunodiffusion and single radial immunodiffusion in agar gel are the qualitative and quantitative tests, respectively, conducted in the exercise. Supplies are suf- ficient for 4 “hands-on” participants. (Some reagents are shared; hot-water bath is required.) Also an excellent demonstration in conjunction with our item #202100 Carolina BioKits®


: Immunodetective Investigation. Note: Contains perishable items;


please give use date on order. 202118 Per kit


100% satisfaction guaranteed Serum Antibody Set


Goat antisera containing immunoglobulin (antibodies) specific for albumin. Suitable for testing ground meat purity.


Goat Anti-Bovine Albumin (1 mL) Understanding Immunology Booklet Goat Anti-Horse Albumin (1 mL)


Instructions


Goat Anti-Swine Albumin (1 mL) 202102 Per set


Teacher’s Choice valuable teacher resources: carolina.com Carolina Exclusive 49 /2 " (selected openings)


Understanding Instructions


Immunology Booklet C


Agar plate demonstrating the double diffusion technique (Ouchterlony analysis) using an animal blood sera set. This plate shows the precipitin line where a specific antigen- antibody interaction has occurred. The antigen (serum albumin) has diffused from the center well in all directions. Antibody (anti-albumin) has likewise diffused from each of the surrounding wells. The homologous antibody and antigen form an insoluble precipitation, the precipitin line, where they meet at critical concentrations. The wells were loaded as indicated below.


Anti-Horse Albumin


Well B Well C


Anti-Swine Albumin


Physiology


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