Cellular ATP & Glycerinated Muscle
A strip of fresh muscle, about 3 mm wide, is dissected and tied to a 120-mm applicator stick. Accessible water-soluble chemicals in the muscle, including ATP, are extracted by cold glycerol. Myofibers retain their striations and can be observed microscopically. The preparation is stored at –10°
ATP Muscle Set • Demonstrate that ATP, potassium ions, and mag- nesium ions are required for muscle contraction
• Observe and measure contraction of muscle fibers
• Observe striated muscle tissue under a microscope
Grade 9–College. Amazingly, glycerinated muscle tissue, although no longer alive, retains the ability to
contract when ATP and the appropriate ions are added. Using these materials, students can observe and measure muscle shortening without a microscope. This activity is designed for minimal prep time and can be completed in 1 class period. The glycerinated muscle used in this set is processed from striated muscle tissue. Much of the water-soluble content of the muscle fibers is extracted in cold glyc- erol. The striations are left intact and are easily observed with a microscope. We prepare the ATP solutions as close to shipping time as practical to reduce loss in chemical activity. When the materials arrive, refrigerate the solutions at 4 to 10°
C
C. The strip of glycerinated muscle can be cut into 2- to 3-cm lengths and is sufficient for 4 to 8 students or groups. With instructions. Note: Perishable; please provide use date on order. Set includes:
and use them within 7 days. Place the tube with muscle strip in a freezer compart- ment at –20 to –10°
Glycerinated Skeletal Muscle Scissors (item #203520)
ATP Solution KCl and MgCI2
Solution ATP, KCl, and MgCI2 203525 Per set
Carolina Teacher Tips Physiological Solutions
Solutions such as Ringer and Iodine-Potassium Iodide are listed alphabetically in the Chemicals listing in the Chemistry section.
Needed but not included: Teasing Needles Millimeter Rulers
Petri Dishes
Solution Microscope Slides and Coverslips (optional) Dissecting and Compound Microscopes (optional)
Rabbit skeletal muscle Glycerinated Skeletal Muscle
Includes glycerinated skeletal muscle in a tube of glycerol as used in our item #203525 ATP Muscle Set. For study of typical skeletal muscle striations and ATP- induced contraction. When it arrives, store the tube with muscle strip in a freezer compartment at –20 to –10°
C. With instructions. 203520 Per tube
Glycerinated Muscle Chemical Solutions Set
The ATP and salt solutions provided with our item #203525 ATP Muscle Set. One set consists of 5 mL each of the 3 solu- tions, each in a dropper vial. On arrival, refrigerate the solutions at 4 to 10°
use them within 7 days. Perishable. 203530 Per set
203530
Glycerinated Muscle Chemical Solutions Set
ATP Muscle Kit Grade 9–College. This activity is designed for minimal prep time and can be com- pleted in 1 class period. Students can observe and measure the contraction of real muscle tissue using the materials included. The kit features glycerinated (preserved) muscle tissue that amazingly retains the ability to contract when ATP and the appropriate ions are added. The glycerinated muscle is processed from striated muscle tissue. During processing, much of the water-soluble content is extracted in cold glycerol, while the striations are left intact and can be easily observed under low-power magnification. We prepare the ATP solutions as close to shipping time as practical to reduce loss in chemical activity. When they arrive, refrigerate the solutions at 4 to 10°
C and use them within 7 days. Place the tubes with muscle strips in a freezer compartment at –20 to –10° C. Each strip of glycer-
inated muscle can be cut into 2- to 3-cm lengths; materials are sufficient for 16 to 32 students. With instructions. Note: Perishable; please provide use date on order. The Replacement Kit includes all * items. Kit includes:
4 Tubes Glycerinated Skeletal Muscle (item #203520)*
4 Dropper Vials ATP Solution* 4 Dropper Vials KCl and MgCI2
Solution*
4 Dropper Vials ATP, KCl, and MgCI2 4× Magnifying Glasses
Microscope Slides and Coverslips 203526 ATP Muscle Kit
203526 ATP Muscle Kit 48 Solution* Each 203527 Replacement Kit Each
carolina.com
Needed but not included: Scissors
Teasing Needles Millimeter Rulers
Dissecting and Compound Microscopes (optional)
C and
C until shipped. We recommend the muscle be cut into 2- to 3-cm lengths for study of striations and contraction. Each strip will thus be adequate for 4 to 8 students.
Physiology
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