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Protein Extraction & Lysis Products


Bacterial PE LB™ Extraction of bacterial and recombinant proteins


For the extraction of biologically active soluble proteins, including recombinant proteins, and inclusion bodies from bacterial cells.


Supplied as a kit, which includes PE LB™ Lysozyme, is a proprietary improvement on the lysozyme based lysis method and results in optimal lysis and minimal contamination. Bacterial PE LB™ buffer is also available separately for further downstream applications.


Insect PE LB™


Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21.


FEATURES


• Provides a simple and versatile method for protein extraction from adherent or suspended Sf9 and Sf21 insect cells


• Compatible with electrophoresis and chromatographic applications APPLICATIONS


• For extraction of soluble proteins from cultured insect cells


• Suitable for most applications including enzyme and protein purification applications, electrophoresis, Western blotting and 2D-gel analysis


Figure 2: Bacteria expressing a His-tagged protein were lysed with Bacterial PE-LB™ and the recombinant protein was purified with HOOK™ 6X His Protein Purification kits (Left: Nickel resin; Right: Cobalt resin). Lane 1: PAGEmark™ protein ladder; 2: Cleared lysate; 3: Flow through; 4-6: Washes; 7-9: Elutions.


FEATURES


• Eliminates mechanical lysis and viscosity build-up • Eliminates removal of nucleic acids


• Suitable for processing 100 x 50μl bacterial cell pellets APPLICATIONS


• Lysis and extraction of proteins from bacterial cells • For the isolation of biologically active proteins


PART NO. 786-176 786-177


DESCRIPTION Bacterial PE LBTM Bacterial PE LBTM Tissue PE LB™


Developed for extraction of total biologically active, soluble proteins from animal tissues.


FEATURES


• Compatible with most enzyme assays including reporter gene assays (ß-galactosidase, luciferase, chloramphenicol acetyltransferase), kinases (protein kinase C, protein kinase A, tyrosine kinase) & immunoassays (ELISA, Western blots, RIA)


APPLICATIONS • Soluble protein extraction from fresh and frozen animal tissue


• Suitable for most applications including enzyme and protein purification applications, electrophoresis, Western blotting and 2D-gel analysis


PART NO. 786-181 786-181T


DESCRIPTION Tissue PE LBTM Tissue PE LBTM


Astral Scientific Product Guide August 2015 SIZE


500ml 50ml


4


Kit including PE LBTM buffer only


SIZE


Lysozyme 100 preps 500ml


PART NO. 786-411


DESCRIPTION Insect PE LBTM


SIZE 250ml Total Protein Extraction (TPE™ FEATURES


• Ready-to-use buffers for extraction of total protein • Two component extraction protocol


• Based on an optimized concentration of Tris and SDS • Supplied with sufficient reagents for 50 x 250mg preparations


PART NO. 786-225


DESCRIPTION Total Protein Extraction (TPETM ) Kit SIZE 50 preps


RIPA Lysis & Extraction Buffer A complete lysis buffer for the release of cytoplasmic, membrane


and nuclear proteins from adherent and suspension cultured mammalian cells.


RIPA Lysis Buffer does not contain protease inhibitors, however it is fully compatible with our range of protease inhibitors cocktails.


PART NO. 786-489 786-490


IBS™ 786-183 DESCRIPTION


RIPA Lysis & Extraction Buffer RIPA Lysis & Extraction Buffer


Buffer


Inclusion bodies solubilization buffer PART NO.


DESCRIPTION IBSTM


Buffer Kit SIZE 100ml SIZE


100ml 500ml


)


For the extraction of total protein from cells & tissues for SDS-PAGE analysis


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