For rapid and sensitive ECL detection in Western Blotting
Highest Dynamic Range
• Attomolar sensitivity • Quantitative - linear range of signal spans 3 orders of magnitude • Low background for optimal signal intensity • Sustained signal allows detection hours after substrate incubation • Compatible with CCD- and x-ray film-based imaging systems
AMRESCO’s VisiGlo™ Prime HRP Chemiluminescent Substrate Kit delivers a wide dynamic range of HRP (horse radish peroxidase) detection that is linear over three orders of magnitude, enabling accurate and quantitative comparison of proteins, especially when combined with CCD imaging. Highly sensitive VisiGlo™ Prime is ideal for detection of low abundance proteins, with sensitivity down to attomolar levels. High abundance proteins are detected without exhibiting substrate depletion, an important feature for simultaneous detection of low and high abundance proteins in a single exposure. The chemiluminescent emission from VisiGlo™ Prime is highly stable, lasting hours after substrate incubation to allow time for multiple exposures to obtain optimal blotting images.
PART NO. AM1B1581-KIT-100ML DESCRIPTION
VisiGlo™ Prime HRP Chemiluminescent Substrate Kit
Sufficient for 1,000 cm2 of membrane SIZE Kit
Figure 10: High sensitivity and low background on a Western blot detected with VisiGlo™ Prime. Serial dilutions of K562 cytoplasmic lysate were resolved by SDS-PAGE and transferred to PVDF using Rapid Transfer Buffer (AMN789). The Western blot was probed with anti-NF-KB (1:2,000) primary and HRP-labeled secondary antibodies. VisiGlo™ Prime HRP Chemiluminescent Substrate detected NF-KB in as little as 625 ng of lysate.
Figure 11: VisiGlo™ Prime provides a long-lasting chemiluminescent signal. A blot containing HRP bands was incubated 5 minutes with VisiGlo™ Prime HRP Chemiluminescent Substrate and imaged at 0, 1 and 3 hours post-substrate incubation using the same exposure settings. Significant chemiluminescent signal was retained even after 3 hours.
Sensitive Substrate for Rapid Results
• Luminol-based substrate for rapid detection of peroxidase-labelled conjugates • Highly sensitive substrate saves precious protein samples and antibodies • Low background without compromising signal intensity • Compatible with CCD- and x-ray film-based imaging systems
AMRESCO’s VisiGlo™ HRP Chemiluminescent Substrate Kit is a luminol-based substrate designed for the rapid and sensitive detection of peroxidase labelled conjugates. It offers increased sensitivity compared to chromogenic substrates in both blotting and ELISA applications. VisiGlo™ can be detected using x-ray film and chemiluminescent imagers in blotting applications or using a luminometer in ELISA. Supplied as kit with equivalent volumes Substrate A and Substrate B for the detection of ~2400 cm2 before use and apply to blot.
of membrane. Mix directly PART NO. AMN218-KIT DESCRIPTION
VisiGlo™ HRP Chemiluminescent Substrate Kit Sufficient for 2,400 cm2
Astral Scientific Product Guide August 2015
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