14 ANALYTICAL AND LABORATORY EQUIPMENT
Incubating the latest developments in IVF
Jacqueline van der Zijden reports on how incubators are currently being used for the cultivation of vital cell lines.
Jacqueline van der Zijden nous présente la façon dont les incubateurs sont actuellement utilisés pour la culture de lignées cellulaires essentielles.
Jacqueline van der Zijden berichtet darüber, wie Inkubatoren derzeit für die Kultivierung lebender Zelllinien verwendet werden.
A
controlled, reliable incubation system can be one of the most
valuable pieces of equipment in a laboratory. Having the ability to culture a healthy cell line can make the difference between successful and failed investigations and influence the results of ground-breaking research projects.
A prime example of this is IVF research taking place at the Karolinska Institute in Sweden, where Panasonic incubators are currently being used for the cultivation of vital cell lines.
IVF procedures Since the birth of the first test tube baby in 1978, the frequency and success of IVF procedures has increased dramatically. A review of 10 reports by international groups estimated that across 74 countries, the number of babies born as a result of the IVF procedure went from around 90,000 to over 2 million between 1990 and 20121
.
Accounting for missing data, it is generally agreed that today there has been more than 5 million IVF births worldwide2
. Following this success, further
improvement of assisted reproduction remains a widely pursued topic. Te continuing development of easier methods has increased interest and introduced opportunities to make IVF more affordable, successful and available to the wider population.
Te Karolinska Institute is the biggest medical university in Sweden and is responsible for some of this research. Te prestigious Institute has a large stem cell programme containing many research groups, one of which is run by Professor Outi Hovatta. Tis project is investigating the maturation of human ovarian follicles and oocytes from cryostored human ovarian tissue. It is hoped that this technique can be employed when there are risks to implanting tissue directly back into the ovary of a woman who has lost her eggs, perhaps due to chemotherapy or genetic causes.
Successfully culturing oocytes from primodial follicles to full maturity is a long and delicate process and can be severely affected by their developing environment. Terefore, when undertaking this painstaking
Fig. 1. Panasonic’s Direct Heat and Air Jacket system.
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