Screening
Discover the effects of a novel compound on a single cell – no longer rely on qualitative methods only
measure a 96-well plate in less than 15 minutes, with less than 0.5% sample carryover. Similarly, its novel FACSymphony cell analyser is designed to leverage the inherent benefits of flow cytometry while enabling the simultaneous measurement of up to 50 different cell characteristics using up to 10 different lasers, thanks to an innovative detection- array technology.
Merck offers a variety of flow cytometer tech- nologies, from compact instruments for basic anal- yses, to more advanced equipment combining flow cytometry with microscopy. Its Muse Cell Analyzer is a compact benchtop-sized instrument suitable for automated cell analysis, capable of analysing three parameters. The Guava easyCyte instrument is a microcapillary-based machine that allows detection of up to 12 parameters, and is capable of handling 96-well plates as well as sample tubes. Merck’s Amnis range of imaging flow cytometers combines the speed and sample size of flow cytometry with the resolution and sensitivity of microscopy in a sin- gle platform. The FlowSight and ImageStreamX are capable of generating qualitative and quantitative imaging data, with both systems allowing up to 12 parameters to be analysed.
The ACEA Biosciences NovoCyte system is a high-performance, cost-effective benchtop flow cytometer designed for all levels of users and all
Drug Discovery World Winter 2017/18
types of laboratories. The instrument is designed for the detection of up to 17 parameters with enhanced sensitivity and resolution, and its fully customisable laser and optical configurations offer a high degree of flexibility while providing com- plex cell analysis. The intuitive build also allows for automated instrument maintenance functions and advanced data analysis capabilities for greater usability. In addition, the NovoSampler Pro autosampler is capable of analysing samples at an acquisition rate of 35,000 cells per second, and is compatible with single tubes, multi-tube racks and 24-, 48- and 96-well plates, with sample volumes ranging from 10µL to 5mL.
Beckman Coulter also offers a range of flow cytometry systems for research and discovery, diag- nostics and industrial applications. The Gallios sys- tem, for example, comes with two to four lasers and can measure eight to 12 parameters. Additionally, its CytoFLEX platform is available in three models – the most advanced possessing up to six lasers, allowing for detection of up to 23 param- eters. The system can also be expanded with a 96- well plate loader, facilitating higher throughput.
The future of the field With simplified, high-speed workflows delivering improved reproducibility and reduced cost per
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References 1 Jepras, R and Ludbrook, S. Evolution of flow cytometry as a drug screening platform, Drug Discovery World, Spring 2013. 2 SLAS 2018 [Internet]. [cited 2017 Nov 28]. Available from:
https://www.slas2018.org/. 3 Saeys, Y, Van Gassen, S and Lambrecht, BN. Computational flow cytometry: helping to make sense of high- dimensional immunology data, Nature Reviews Immunology, 2016, 16, 449-462. 4 Kolontaj, K et al. Automated nanoscale flow cytometry for assessing protein-protein interactions, Cytometry A, 2016, 89, 835-843.
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