Screening
ed set-up was able to analyse 4000 FRET samples within two days on a single flow cytometer.
Commercially-available flow cytometry platforms
There are currently a wide variety of flow cytome- try systems on the market, available from a num- ber of different manufacturers.
The Intellicyt iQue Screener PLUS platform is an integrated software and reagent system that enables rapid, high-content, multiplexed analysis of cells and beads in suspension. The platform comes in three different configurations, utilising either two or three lasers, which allows for up to 13-colour channel detection, ideal for functional and phenotypic applications that require more flexibility in experimental design. By maximising the detection and resolution of traditional and innovative reagent dyes, tandem dyes and fluores- cent proteins, the iQue Screener PLUS platform delivers high-performance, multicolour analysis, and is able to measure up to 35,000 cells per sec- ond. The system is designed for use with 96-, 384- and 1536-well plates, and users can work with as little as 1µL of sample. Miltenyi Biotec’s MACSQuant range of flow cytometers combines performance and robustness with highest convenience. The range includes the MACSQuant Analyzer 10, MACSQuant VYB and MACSQuant X systems. Utilising three lasers (405, 488 and 638nm), the compact, robust 10-
Take drug discovery forward, target the right cell at the right time to identify new lead- candidates for your pipeline
parameter MACSQuant X flow cytometer is designed to facilitate the running of 10-parameter experiments in just 15 minutes for a 96-well plate, and less than 60 minutes for a 384-well plate. The system comes with an integrated multisample anal- yser that can analyse up to 15,000 cells per sec- ond – making it well-suited for high-throughput applications – and is designed to produce consis- tent results with less than 0.01% carryover between samples.
BD Biosciences offers a wide range of flow cytometers for research and clinical diagnostics applications. The BD Accuri C6 PLUS is designed with ease of use in mind allowing both new and experienced flow cytometer researchers to benefit from enhanced sensitivity and reliability in their experiments. The system comes with two in-built lasers, meaning up to four fluorophores can be used at any one time. For walkaway convenience, the BD Accuri CSampler Plus automation accesso- ry automates sample handling for applications using 48- and 96-well plates and 24-tube racks and is designed to be used in combination with the Accuri C6 PLUS.
The BD LSRFortessa X-20 cell analyser, which can be fitted with up to five lasers enabling the detection up to 20 parameters simultaneously, is designed to deliver high-performance multicolour analysis with an extremely compact footprint (30” by 20”). With its fully automated sample handling functionality, the LSRFortessa X-20 analyser can
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Drug Discovery World Winter 2017/18
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