Product Profile


Gene expression analysis by RNA in situ hybridisation


Get quantitative molecular detection with morphological context in a single assay R


NAscope® assay is the most advanced RNA in situ hybridisation (ISH) assay based on ACD’s proprietary technology with signal amplification and simultaneous background noise suppression which advances biomarker analysis in tissues and cells. Unique to this technology, the RNAscope® assay delivers quantitative, sensitive and specific molecular detection of RNA species on a cell- by-cell basis with morphological context in a robust assay. This enables researchers to visualise which genes are expressed, localise where they are expressed, and quantify the level of expression down to a single transcript of RNA. Learn more at www.acdbio.com.


Detection of lncRNA


Detection of PCA3 in human prostate cancer tissue using RNAscope® 2.5 HD Brown Assay


Detection of SChLAP1 in human prostate cancer tissue using RNAscope® 2.5 HD Brown Assay


Detection of lncRNA in normal mouse brain using the RNAscope® Multiplex Fluorescent assay on fresh frozen tissue samples. Image overlay mouse brain: NEAT1 (Red), MALAT1 (Green), H19 (White), Cells counterstained with DAPI, CA = Hippocampus Cornu Ammonis, DG = Hippocampus Dentate Gyrus


Detection of cytokines


Detection of TNF- in human breast cancer tissue using RNAscope® 2.5 HD Brown Assay


Other examples


Detection of IFN- (red) and CD8a (green) in human non–small cell lung cancer using the RNAscope® 2.5 HD Duplex Assay


Detection of CXCL13 in human stomach cancer using the RNAscope® 2.5 HD Brown Assay


Detection of LGR5 in human colon cancer using RNAscope® 2.5 HD Brown Assay


Detection of FGFR2 in human lung tissue using RNAscope® 2.5 LS Reagent Kit-RED


Detection of the three distinct Opioid G protein- coupled receptor types in the normal mouse brain using the RNAscope® Multiplex Fluorescent assay on


Fresh Frozen tissue samples: Opioid Receptor µ (OPRM1), δ (OPRD1) and κ (OPRK1). Image overlay mouse brain: OPRM1 (Green), OPRD1 (Red), OPRK1


(White), Cells are counterstained with DAPI, DG = Dentate Gyrus


Drug Discovery World Winter 2017/18 15


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