Stem Cells


Figure 1


Human-induced pluripotent stem cells (iPSCs) possess a number of important


properties that make them an excellent source of cells for


disease models and compound screening assays. The image


illustrates Axol’s Human iPSC- derived Neural Stem Cells forming a rosette structure.


Markers used: acetylated alpha tubulin (green), CEP135 (red), DAPI (blue)


Quinn, Associate Marketing Director for Stem Cell Technologies at Takara Bio. “Now, some ven- dors are commercialising complete systems for the differentiation of cells into various disease-rele- vant cell types, such as hepatocytes and beta cells. As a result, we should expect to see the publica- tion of more differentiation protocols that will allow for scale-up on a level that will facilitate drug screening.”


Commercially-available stem cell technologies Many technology developers are heavily engaged in the stem cell field and working to overcome cur- rent challenges and realise the full potential of stem cells for drug discovery.


Agilent’s Seahorse XF technology enables reliable prediction, monitoring and tracking of cell fate transitions to accurately identify pluripotency and differentiation transitions. Agilent Seahorse XF Analyzers simultaneously measure mitochondrial


48


and glycolysis energy pathways in live cells using label-free,


solid-state sensor cartridges


in


microplate format. Together with the Seahorse XF Assays and Kits range, which includes the XF Cell Mito Stress Test Kit and XF Glycolytic Rate Assay Kit, this platform can be used to take metabolic measurements by which reprogramming efficiency, differentiation and lineage commitment can be determined.


Axol Bioscience has a broad range of products for stem cell research, including a large number of iPSC-derived cells that comprise neural, cardiac, vascular, immune and renal cells, among others. Axol also offers an extensive collection of human iPSC-derived disease models, including those for Alzheimer’s disease, Huntington’s disease, fron- totemporal dementia, epilepsy and a wide range of cancers. Isogenic human iPSCs, developed by introducing disease-associated mutations into cell lines using CRISPR-Cas9 gene editing, are also available (Figure 1).


Drug Discovery World Fall 2018


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