Pharmaceutical & medical The flow port adaptor has
been designed to allow CO2 gas to be exchanged inside the gas measurement chamber.
Both series of sensors are available with CO2 concentration ranges from 0-5 per cent up to
0-100 per cent. They feature a typical measurement accuracy of 70ppm and have built in auto-zeroing, ensuring excellent long- term accuracy.
pH value, which is typically in the range from 7.0 to 7.7. Chemical reactions within the medium can alter the pH. In order to counter
these reactions, levels of CO2 are controlled, helping to maintain a steady pH equilibrium in the growth medium.
If there is not enough CO2 in the atmosphere, CO2 will outgas from the culture media, and the substance will become
too alkaline; excessive CO2 concentration in the atmosphere will cause more CO2 gas to
be absorbed by the mixture, leading to high
acidity levels. Providing a controlled CO2 atmosphere helps maintain the required
concentration of CO2 in the growth medium, stabilising the resultant pH.
WHAT ARE THE REQUIREMENTS FOR THE CO2 SENSOR? A CO2 sensor used in a cell incubator should be responsive, accurate and stable over time.
Undesired changes within the incubator are likely to have a negative impact on the culture, and it is therefore vital to know when
CO2 levels have changed, as well as the extent of the variation and to be able to respond accordingly. Due to the high humidity environment, action also needs to be taken to mitigate the effects of high humidity levels particularly during the sterilisation process. Condensation on the sensor will cause the
sensor to report inaccurate results.
Having a fast CO2 sensor that can detect a change in CO2 gas levels enables adjustments to be made in near real-time, reducing the
opportunity for the incubator environment to drift far from nominal.
NDIR CO2 SENSORS Gas Sensing Solutions’ CO2 sensors use a solid state, LED light source. This type of light
source has numerous advantages, particularly for applications that need high response
times. The CO2 gas is strongly absorbed by mid-IR 4.25um light. GSS manufactures its own LEDs, which have been specifically designed to emit a narrowband of light centred at 4.25um.
GSS has designed a family of high speed,
high response rate CO2 sensors specifically targeted at applications that require near real- time CO2 measurement capability.
INTRODUCING THE SPRINTIR-6S AND EXPLORIR-W The SprintIR-6S and ExplorIR-W series of sensors are a range of high accuracy carbon
dioxide (CO2) sensors designed to meet the needs of customers who require fast
measurement of CO2 levels. The sensors are designed to take a reading 20 times a second for the SprintIR-6S and two times a second for the ExplorIR-W. The optical cavity has been designed along with a flow port adaptor to enable gas to flow through the sensor at
The ExplorIR -W CO2 sensor from Gas
high speed, enabling the user to make use of the very high measurement rate capability. The flow port adaptor has been designed
to allow CO2 gas to be exchanged inside the gas measurement chamber.
Both series of sensors are available with CO2 concentration ranges from 0-5 per cent, up to 0-
100 per cent. They feature a typical measurement accuracy of 70ppm and have built in auto-zeroing, ensuring excellent long-term accuracy. In most applications, with a measurement
rate of up to 20Hz, the ability of the sensor to provide real-time readings will be limited by the flow rate through the sensor. The most important factor is the gas exchange rate. This is the amount of time it takes for the gas to
enter the CO2 measurement chamber, get measured and then replaced. The sensor has a gas measurement chamber volume of approximately 2.8ml. As a rule, to properly exchange the gas in the chamber, there needs to be a x5 volume of gas passed through the sensor. Therefore, approximately 14ml of gas needs to flow through the sensor for each reading to be effective.
The maximum flow rate supported by the
SprintIR-6S is approximately 19ltr/minute CO2 before the sensor is unable to track the gas flow.
The SprintIR-6S and ExplorIR-W CO2 sensors from GSS have been specifically designed to address applications where high measurement rate, responsiveness and long-term accuracy are paramount.
Suitable for CO2 incubators and a variety of other life science applications, they come with several options that allow the sensor to be optimised for the installation.
Gas Sensing Solutions www.gassensing.co.uk
Instrumentation Monthly June 2020 17
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