NIGHTSEA Stereo Microscope Fluorescence Accessories (continued) White Light Head


This is an extremely convenient option for general illumination and as a focusing aid for fluorescence imaging. Now if you


are using the NIGHTSEA system for fluorescence you do not need a separate white light source. Just exchange the fluorescence excitation light head module for the white-light module in a matter of seconds.


Cat No. SFA-LH-WH Description Modular White Light Head Infrared Light Head


Researchers sometimes need an infrared light source for non- intrusive observation of behavior. And sometimes the subjects are small and you need to make the observations with a stereo microscope. The SFA-LH-IR850 is an 850nm light source that is compatible with the NIGHTSEA Model SFA Stereo Microscope Fluorescence Adapter. The light head plugs directly into the lamp base supplied with the SFA system. You can acquire the light head as a supplement to the SFA system that you already own for fluorescence observation, or you can acquire it in combination with a lamp base as a stand-alone device.


850nm IR Light Head


Qty. each


Focus Lens for SFA Light Head


The focus lens is an add-on for the SFA light heads to produce a higher illumination intensity! This is a simple adapter that slips over the front of your existing NIGHTSEA SFA light head and grips with a friction fit. It is available with two options for the focal distance: 30mm or 50mm.


No lens – illumination spot approximately 15mm diameter with the light head positioned 50mm from the surface.


50mm lens – central spot approximately 5mm diameter, intensity increase ~2x.


850nm IR Light Head on SFA Lamp Base


30mm lens – central spot approximately 2mm diameter, intensity increase ~3.5x.


The images below show the focus lens add-on and the effect. The three illumination spot images were made in manual exposure mode and are indicative of the relative intensity.


The light head incorporates a high power 850nm LED and a medium beamwidth diffusing lens to create a smooth illumination area. The output is centered at 850nm with a FWHM (full width at half maximum) of approximately 50nm. There is virtually no emission at wavelengths shorter than 750nm.


We recommend that you use the DIM base with the LH-IR850 in order to have finer control of illumination intensity.


A test target with circles of 2, 5, and 10cm diameter.


No Focus Lens


50mm Focus Lens Considerations for lens choice:


To gain the benefit of the 30mm lens it is necessary to position the light head very close to the subject. This may be challenging in the tight space beneath a microscope objective. It might be more suited for photoactivation, where a high light intensity dose in a small area is often needed.


The 50mm lens provides a trade-off – brighter fluorescence, easier to work with.


Note – If the light head has a snoot installed that will have to be removed to use the focus add-on.


Cat No. Description


SFA-FL-30 Focus Lens for SFA Light Head with 30mm Lens SFA-FL-50 Focus Lens for SFA Light Head with 50mm Lens


The photo on the left above was taken with a conventional camera under normal room light, and with the IR light head illuminated at full power, directed at the target center from a distance of approximately 10cm (4 in.). The illumination spot is not visible either to this camera or to the naked eye. The photo on the right was made under the same lighting conditions, but with a camera that had been modified to image only infrared light.


Please note that infrared light is not visible to the human eye and to many cameras. You will need an appropriate camera to record the observations.


Cat No.


SFA-LH-IR850 SYS-IR850


Description IR Light Head


IR Light Head plus NIGHTSEA DIM Base


Qty. each each


7


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Qty. each each


30mm Focus Lens


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