NIGHTSEA Model SFA Fluorescence Adapter for Hirox (continued)
Why we recommend a set of three excitation wavelengths for industry needs
We have found that the combination of the Ultraviolet, Violet, and Royal Blue excitation/emission sets addresses a wide variety of industrial imaging challenges. The most common misconception we encounter is that fluorescence is uniquely associated with ultraviolet (UV) light and many customers immediately ask for that wavelength. However, we have found in several cases – even some in which UV was specifically recommended by a dye manufacturer – that either Violet or Royal Blue provided superior performance. For example:
A prospective customer wanted to image concrete thin sections impregnated with fluorescent dye. The dye supplier recommended UV, and it is true that UV will make the dye fluoresce nicely. We found that our Royal Blue excitation produced significantly brighter fluorescence.
Similar to above, except that a fluorescence epoxy had been used to highlight surface cracks in a test sample. Royal Blue excitation yielded brighter fluorescence.
Acquiring the three-color system will provide the highest chance of success and will equip you with a versatile toolkit to address new fluorescence imaging challenges as they arise. Contact us if you would like to discuss your application further.
In addition to the Comprehensive Solution Kits, you can also purchase single-color full systems that provide everything you need to get started with one excitation/emission combination. Once you have a full system, additional wavelength sets can be ordered separately. These contain the new light head, microscope barrier filter, and barrier filter glasses.
Wavelength Sets
There are five excitation/emission wavelength combinations available for the system.
Color
Ultraviolet Violet
Royal Blue Cyan
Green
Designation UV VI
RB CY GR
Excitation
360 - 380nm 400 - 415nm 440 - 460nm 490 - 515nm 510 - 540nm
Emission
415nm longpass 450nm longpass 500nm longpass 550nm longpass 600nm longpass
Ordering Information Cat. No.
Description
Best Value Full System Kit for HR-1020E Lens Includes three wavelengths (UV + VI + RB) SFA-HR1020E-IND
HR-1020E Best Value Full System
Single Wavelength Full Systems for HR-1020E Lens SFA-HR1020E-UV SFA-HR1020E-VI SFA-HR1020E-RB SFA-HR1020E-CY SFA-HR1020E-GR
Qty. kit
HR-1020E Single Wavelength Full System, UV HR-1020E Single Wavelength Full System, Violet HR-1020E Single Wavelength Full System, Royal Blue kit HR-1020E Single Wavelength Full System, Cyan HR-1020E Single Wavelength Full System, Green
kit kit
kit kit
Light + Filter Sets for HR-1020E Lens SFA-HR1020E-LFS-UV HR-1020E Light + Filter Set, UV Excitation SFA-HR1020E-LFS-VI HR-1020E Light + Filter Set, Violet Excitation
Barrier Filters for HR-1020E Lens SFA-HR1020E-BF-UV HR-1020E Barrier Filter, UV HR-1020E Barrier Filter, Violet
SFA-HR1020E-BF-VI
SFA-HR1020E-BF-RB HR-1020E Barrier Filter, Royal Blue SFA-HR1020E-BF-CY HR-1020E Barrier Filter, Cyan SFA-HR1020E-BF-GR HR-1020E Barrier Filter, Green
Cat. No. Description
Best Value Full System Kit for HR-2500E Lens Includes three wavelengths (UV + VI + RB) SFA-HR2500E-IND
HR-2500E Best Value Full System
Single Wavelength Full Systems for HR-2500E Lens SFA-HR2500E-UV SFA-HR2500E-VI SFA-HR2500E-RB SFA-HR2500E-CY SFA-HR2500E-GR
each each
SFA-HR1020E-LFS-RB HR-1020E Light + Filter Set, Royal Blue Excitation each SFA-HR1020E-LFS-CY HR-1020E Light + Filter Set, Cyan Excitation SFA-HR1020E-LFS-GR HR-1020E Light + Filter Set, Green Excitation
each each
each each each each each
Qty. kit
HR-2500E Single Wavelength Full System, UV HR-2500E Single Wavelength Full System, Violet HR-2500E Single Wavelength Full System, Royal Blue kit HR-2500E Single Wavelength Full System, Cyan HR-2500E Single Wavelength Full System, Green
kit kit
kit kit
Light + Filter Sets for HR-2500E Lens SFA-HR2500E-LFS-UV HR-2500E Light + Filter Set, UV Excitation SFA-HR2500E-LFS-VI HR-2500E Light + Filter Set, Violet Excitation
Barrier Filters for HR-2500E Lens SFA-HR2500E-BF-UV HR-2500E Barrier Filter, UV HR-2500E Barrier Filter, Violet
SFA-HR2500E-BF-VI
SFA-HR2500E-BF-RB HR-2500E Barrier Filter, Royal Blue SFA-HR2500E-BF-CY HR-2500E Barrier Filter, Cyan SFA-HR2500E-BF-GR HR-2500E Barrier Filter, Green
Gel defect in a nylon granule
Nylon 6,6 granules can manifest a process defect called “gel” and if there is too much of this in a production batch it can compromise downstream production. The gel shows up as a brighter fluorescent area within the fluorescing granule. Images made with Royal Blue excitation.
For other Hirox lenses... Cat. No.
Description
Best Value Full System Kit Includes three wavelengths (UV + VI + RB) SFA-H-IND
Single Wavelength Full Systems SFA-H-UV SFA-H-VI SFA-H-RB SFA-H-CY SFA-H-GR
Nylon granules with gel defect, 20x, white light and fluorescence under Royal Blue excitation.
Add-on Single Wavelengths SFA-H-LFS-UV SFA-H-LFS-VI SFA-H-LFS-RB SFA-H-LFS-CY SFA-H-LFS-GR
Hirox Best Value Full System
Hirox Single Wavelength Full System - UV Hirox Single Wavelength Full System - Violet
each each
SFA-HR2500E-LFS-RB HR-2500E Light + Filter Set, Royal Blue Excitation each SFA-HR2500E-LFS-CY HR-2500E Light + Filter Set, Cyan Excitation SFA-HR2500E-LFS-GR HR-2500E Light + Filter Set, Green Excitation
each each
each each each each each
Qty. kit
kit kit
Hirox Single Wavelength Full System - Royal Blue kit Hirox Single Wavelength Full System - Cyan Hirox Single Wavelength Full System - Green
kit kit
Hirox Single Wavelength Add-On - UV Hirox Single Wavelength Add-On - Violet Hirox Single Wavelength Add-On - Royal Blue Hirox Single Wavelength Add-On - Cyan Hirox Single Wavelength Add-On - Green
each each each each each
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