NIGHTSEA Model SFA Fluorescence Adapter for Keyence VHX


Overview


The NIGHTSEA Model SFA Fluorescence Adapter system adds a versatile fluorescence imaging capability to the Keyence VHX series of digital microscopes. The system is being used successfully by a growing number of Keyence owners for a variety of applications. Visit our website for a full gallery of images made with the NIGHTSEA adapter and the Keyence microscope.


Fluorescence solutions for most Keyence lenses


Keyence offers a variety of lenses for imaging at different scales. NIGHTSEA offers several variants of our fluorescence adapter system that work with the most commonly used Keyence lenses – the VHX-7100, VH-ZST, -Z100, -Z50, -Z20, and -Z00.


The key elements of any fluorescence system are:


n A light source that produces sufficient energy in the appropriate wavelength range to excite fluorescence in the sample of interest


n A barrier filter in the viewing path that blocks reflected excitation light while transmitting the fluorescence emitted by the sample


NIGHTSEA implements these for the Keyence system with:


n high intensity LED light sources available in five excitation wavelength ranges (see list on next page)


n emission barrier filters that can be added to the Keyence lenses easily and non-invasively


The components of a NIGHTSEA system that adds a single excitation/emission wavelength combination on a Keyence microscope are:


n Flexible gooseneck lamp base with LED drive circuit and intensity control; n Universal power supply (120/240VAC, 50/60Hz) with international plug set n Excitation light source n Emission barrier filter n Barrier filter glasses for fluorescence viewing and eye safety n Padded carrying case


Additional wavelength sets are implemented by adding a matched set (light plus filter set) consisting of a light source, barrier filter, and filter glasses.


Fluorescent mineral sample imaged with the Keyence + NIGHTSEA systems


Comprehensive Solution Kit


We have configured Keyence fluorescence adapter kits that include the three most useful excitation wavelengths for varied industry applications, and that are suited to various combinations of the supported Keyence lenses. The kit price is significantly less than purchasing the items separately.


Custom case for 3-color system for Keyence


Each Industry Kit includes the following items, or a subset that is suited to the particular Keyence lenses you want to outfit:


• Three excitation light heads – Ultraviolet (UV), Violet (VI), and Royal Blue (RB) This recommendation is based on our experience with varied applications. Read the explanation below.


• Three barrier filters, of one or more types depending on the lens(es) to be supported


• Matching barrier filter glasses for each excitation wavelength


• *Accessory hardware for mounting the NIGHTSEA light head over the VH- ZST fiber optic input port (enhanced performance at high magnification)


• Gooseneck lamp base with dimming control • Universal power supply – 120/240VAC, 50/60Hz, with international plug set • Packing/carrying case with custom-cut foam * Only included with systems that support the VH-ZST lens


Here are samples of what you can see:


Naturally fluorescent mineral


This mineral sample, collected at the Sterling Hill Mine in Ogdensburg, New Jersey, contains willemite (green fluorescence), calcite (red fluorescence) and franklinite (black–no fluorescence).


10


Cement thin section fluorescence VH-Z100 lens, Royal Blue excitation, 700x


Epoxy on motor shaft, VH-ZST lens, Ultraviolet excitation, 20x


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24