NIGHTSEA Model SFA Fluorescence Adapter for Keyence VHX (continued) Lens-specific solutions


The implementation approaches for the supported Keyence lenses are summarized here.


VH-Z00, -Z20, -Z50 Light source – placed to the side of the microscope stage


Barrier filter – slips over the bottom of the lens and attaches with thumbscrews. See at right. VH-Z100


Light source – placed to the side of the microscope stage or mounted over the Keyence light source input port


Barrier filter – inserts in the Analyzer slot at the top of the lens


VH-Z100 lens light input port with NIGHTSEA adapter and light head.


Z00, Z20, and Z50 lenses – the barrier filter slips over the bottom of the lens.


Why we recommend a set of three excitation wavelengths for industry needs


Z100 and ZST lenses – the barrier filter inserts in the Analyzer slot at the top of the lens.


VH-ZST • 20-200 lens


Light source – placed to the side of the microscope stage


Barrier filter – inserts in the Analyzer slot at the top of the lens


• 200-2000 lens


Light source – mounts over the Keyence fiber optic input port (except for UV) providing enhanced performance at high magnification


Barrier filter – inserts in the Analyzer slot at the top of the lens


VHX-7100 Fully Integrated (FI) Head • VHX-E20 and -E100


Light source – placed to the side of the microscope stage


Barrier filter – inserts in the Analyzer slot at the right side of the FI head


• VHX-E500 and -E2500 Use with these lenses is likely not practical due to short working distance


NIGHTSEA barrier filter inserted in FI head Analyzer slot


Keyence VH-ZST lens, fiber optic port with NIGHTSEA adapter and light head


We have found that the combination of the Ultraviolet, Violet, and Royal Blue excitation/emission sets addresses a wide variety of industrial imaging challenges. The most common misconception we encounter is that fluorescence is uniquely associated with ultraviolet (UV) light and many customers immediately ask for that wavelength. However, we have found in several cases – even some in which UV was specifically recommended by a dye manufacturer – that either Violet or Royal Blue provided superior performance. For example:


A prospective customer wanted to image concrete thin sections impregnated with fluorescent dye. The dye supplier recommended UV, and it is true that UV will make the dye fluoresce nicely. We found that our Royal Blue excitation produced significantly brighter fluorescence.


Similar to above, except that a fluorescence epoxy had been used to highlight surface cracks in a test sample. Royal Blue excitation yielded brighter fluorescence.


A prospective customer sent us a sample of material that was known to fluoresce under UV. The goal was to see the distribution of non-fluorescent particles in a fluorescent matrix. While both UV and Royal Blue excited fluorescence in the sample, the best imaging contrast was achieved with Violet excitation.


We achieve enhanced performance with the ZST lens by mounting the light head on the lens's fiber optic port, but that path does not transmit UV well. In many cases, for example to inspect conformal coating, Violet is an excellent alternative to UV.


Acquiring the three-color system will provide the highest chance of success and will equip you with a versatile toolkit to address new fluorescence imaging challenges as they arise. Contact us if you would like to discuss your application further.


Wavelength Sets Color


Ultraviolet Violet


Royal Blue Cyan


Green


Designation UV VI


RB CY GR


Excitation


360 - 380nm 400 - 415nm 440 - 460nm 490 - 515nm 510 - 540nm


Emission


415nm longpass 450nm longpass 500nm longpass 550nm longpass 600nm longpass


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