NIGHTSEA Stereo Microscope Fluorescence Adapter (continued)


Once you are set up for one excitation/emission wavelength combination, additional combinations can be added by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.


Wavelength Sets Designation UV – Ultra Violet


VI – Violet RB – Royal Blue


Excitation Emission 360-380nm 415nm LP


400-415nm 450nm LP 440-460nm 500nm LP


490-515nm 550nm LP 510-540nm 600nm LP


Fluorophores DAPI, ...


CFP, ...


RB-GO – Green Only 440-460nm 500-560nm BP GFP, eGFP, fluorescein... CY – Cyan GR – Green


DsRed, dTomato... Green-Only Barrier Filter


The Green-Only (GO) Barrier Filter isolates the green part of the spectrum and is for use with the Royal Blue excitation source. While our other barrier filters are longpass filters this filter is a bandpass, transmitting from approximately 500 to 560nm. The longpass filter has served well for most users who need to visualize green-fluorescent protein (GFP), and if you are exploring fluorescence in nature it is preferable. The primary motivation for adding the green-only filter to the line-up was for the benefit of researchers using GFP in plants such as Arabidopsis thaliana, a common research model. Plants contain chlorophyll, which has a distinctive red fluorescence that can sometimes mask the GFP emission, making it harder to see and photograph.


GFP, eGFP, fluorescein... YFP, Venus, Lucifer Yellow...


Lamp Base Light Control Options


The SFA lamp base is available in three versions: Standard, DIM, and PULSE. Standard lamp bases have a simple OFF/ON operation. DIM lamp bases feature an OFF/ON/DIM switch to change the intensity of the light. PULSE lamp bases incorporate a BNC connector that accepts a voltage signal to control the excitation source ON/OFF.


Only one control option is available per base. The DIM and PULSE options can be purchased as part of a new system or in a stand-alone lamp base purchased a la carte. The PULSE option can be retrofitted to an existing base.


NIGHTSEA DIM Option


The DIM option adds a combined switch and dimmer control. When you first turn the unit on it is at full power. As you rotate the switch the intensity decreases, reaching about 30% power at the minimum setting. You have finer control in the brighter portion of the adjustment range. An intensity indicator (1-10) makes it easy to record and repeat preferred settings.


Dimmer switch option on SFA


Target at max and min excitation NIGHTSEA PULSE Option


The PULSE option adds a BNC connector on the rear of the base that accepts a user- supplied voltage input to turn the light on and off. Some possible applications are:


Arabidopsis fluorescence imaged with longpass filter


Arabidopsis fluorescence imaged with bandpass filter


• External control (computer, function generator, etc.) for precise illumination timing for photoactivation, behavioral experiments


• PWM input for light dimming Voltage Input


Maximum Operation Frequency BNC cable not included


Arabidopsis fluorescence imaged with longpass filter


Arabidopsis fluorescence imaged with bandpass filter


We tested this new barrier filter with Arabidopsis supplied by Dr. Chip Celenza (Department of Biology, Boston University). These plants express GFP in the roots and vasculature. The images above show examples of plants photographed with the longpass filter (left) and green-only filter (right). There is no chlorophyll in the roots so the GFP is evident there in both images, but the weaker expression in the leaves is much more apparent in the images on the right.


See how it works... Learn how to do it...


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2.8 - 6.0 VDC for ON, <0.6VDC OFF 10kHz


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