NIGHTSEA Applications


Screening FISH-Labeled Planarian (Schmidtea mediterranea)


FISH (fluorescence in situ hybridization) is routinely used to label features in planarians (Schmidtea mediterranea). The NIGHTSEA Model SFA Stereo Microscope Fluorescence Adapter can be added to just about any existing stereo microscope to create a practical system for screening samples for successful preparation prior to moving to higher resolution imaging techniques.


Opsin and wntP-2 labeled with FITC (fluorescein isothiocyanate). Specimen courtesy of Lauren Cote, Reddien Laboratory, Whitehead Institute, MIT.


GFP C. elegans mCherry C. elegans Fluorescing C. elegans


The pictures of fluorescing transgenic C. elegans in this gallery were all taken using the NIGHTSEA Stereo Microscope Fluorescence Adapter.


NB.22.1e labeled with rhodamine, highlighting the mouth and the dorsal- ventral boundary. Specimen courtesy of Lauren Cote, Reddien Laboratory, Whitehead Institute, MIT.


YFP C. elegans Fluorescing Xenopus


All of the specimen photographs below were taken with a Canon Rebel T2i camera mounted on a Motic trinocular stereo microscope with the NIGHTSEA Stereo Microscope Fluorescence Adapter for illumination and filtering.


YFP C. elegans


Fluorescent Axolotl


These pictures of GFP-expressing transgenic neurula stage axolotl (Ambystoma mexicanum) embryos were taken using the NIGHTSEA Stereo Microscope Fluorescence Adapter for illumination, with an iPhone 5 camera held up to the eyepiece. It’s a convenient way for students to take photos during labs! Access to specimens courtesy of Dr. Kristi Wharton and Kathy Patenaude, Brown University.


Stage 41 X. tropicalis, transgenic OTX-GFP eyes.


Stage 29-30 X. laevis, messenger RNA injected ubiquitous GFP and membrane RFP.


Axolotl (Ambystoma mexicanum), white light.


Axolotl (Ambystoma mexicanum), fluorescence.


Collection of Stage 37-38 X. laevis, messenger RNA injected ubiquitous GFP and membrane RFP viewed through shield filter for sorting.


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Collection of Stage 37-38 X. laevis, messenger RNA injected ubiquitous GFP and membrane RFP viewed through shield filter for sorting.


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