Radial Immunodiffusion


Radial Immunodiffusion (RID) is based on the complexing of antigen and antibody to produce a visible precipitin ring.


BINDARIDTM


technology. NANORIDTM


kits use conventional antibody-antigen binding RID


kits use a novel patented technology to enable accurate quantitation of very low concentrations of protein in biological materials.


The procedures for three different methods are included in the instructions: 1. RID Reference Table (Mancini) 2. Complete Diffusion (Mancini) 3. Incomplete Diffusion (Fahey and McKelvey)


Human Complement Proteins DESCRIPTION PACK C1 Inactivator - NL RID kit


Range 45-450 mg/L C1q - NL RID kit


Range 23-230 mg/L** C2 - NL RID kit


Range 7.2-36 mg/L C3 - NL RID kit


Range 155-1550 mg/L C4 - NL RID kit Range 58-580 mg/L


CODE 3 plate kit RN019.3 3 plate kit RN020.3 3 plate kit RN022.3 3 plate kit RN023.3 3 plate kit RN025.3


C4 - Binding Protein - NL RID kit 3 plate kit RN026.3* Range 50-500 mg/L C5 - NL RID kit


3 plate kit RN027.3


Range 20-200 mg/L C6 - NL RID kit


Range 12-120 mg/L C7 - NL RID kit


Radial immunodiffusion kit with RID plate reader RID Kit Contents


• RID Plates - 14 pre-cut wells per plate. Gel sectioning blades allow fewer than 14 wells to be used.


• Calibrators - Each kit contains either a single high calibrator or a 3 calibrator set.


• Controls - Control material is include in the majority of kits.


• Sample Diluent - Used to dilute samples, controls and calibrators where necessary; eliminates the possibility of viscosity differences causing inaccurate or incorrect results.


• Instruction Leaflet and Results Table - Full instructions are included together with a RID Reference Table containing protein concentration values for specific ring diameters.


Human Complement Functional Assays


Functional assays are effective as screening tools to detect complement deficiencies and aid in the monitoring of total complement activity. Assays are based on the haemolysis of red blood cells following activation of the complement system.


DESCRIPTION PACK CODE


Total Haemolytic Complement kit 3 plate kit RC001.3 Maximum 12 weeks from manufacturing 2 plate kit RC001.2 to expiry


1 plate kit RC001.1


Alternative Pathway Haemolytic kit 3 plate kit RC003.3* Maximum 6 weeks from manufacturing to expiry


1 plate kit RC003.1* Functional C1 Inactivator kit


Maximum 12 months from manufacturing to expiry


Functional C1 Inactivator COMBI kit 3 plate kit RK019 Two plates of Functional C1 Inactivator One plate of C1 Inactivator


Maximum 12 months from manufacturing to expiry


3 plate kit RC002.3 Total Haemolytic Complement plate after haemolysis. * For research use only.


See page 19 for the CH50 Eq ELISA kit and page 8 for the CH50 SPAPLUS turbidimetric assay.


Range 22-110 mg/L C8 - NL RID kit


Range 20-200 mg/L C9 - NL RID kit


Range 50-500 mg/L Factor B - NL RID kit


Range 45-450 mg/L Factor H ( 1H) - NL RID kit


Range 70-700 mg/L Factor I - NL RID kit Range 7-70 mg/L


Human Coagulation Proteins DESCRIPTION PACK Antithrombin III - NL RID kit


Range 50-500 mg/L Fibrinogen - NL RID kit


Range 450-4500 mg/L Plasminogen - NL RID kit Range 20-200 mg/L


Protein C RID kit Range 0.5-5.0 mg/L CODE 3 plate kit RN040.3 3 plate kit RN056.3 3 plate kit RN065.3 3 plate kit GT118.3 3 plate kit RN102.3* 3 plate kit RN103.3* 3 plate kit RN089.3* 3 plate kit RN028.3* 3 plate kit RN029.3 3 plate kit RN030.3* 1 plate kit RN031.1*


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