IgG Subclasses 


For the reliable measurement of subclass concentrations Binding Site assays utilise highly specific, affinity purified, polyclonal antisera raised in sheep using patented technology. Manufacturing date to expiry is maximum 18 months unless otherwise stated.


Sensitivity


Binding Site IgG Subclass assays are optimised for use in the investigation of antibody deficiencies. Latex-enhanced reagents are provided for the measurement of IgG3 and IgG4 enabling accurate quantitation of deficient subclass levels as well as levels at the lower end of paediatric normal ranges.


Standardisation


Calibration of an assay against an internationally recognised reference preparation will ensure that sample results remain accurate and consistent. In 1997 Carr-Smith et al. assigned IgG subclass values to the international serum protein reference material CRM470 which is the most commonly used reference material for commercial IgG assays. All Binding Site IgG subclass assays were subsequently calibrated against CRM470, with conversion factors available for customers wishing to compare results with those obtained in assays calibrated against the much earlier reference material WHO67/97 which is no longer available.


More recently a new international reference material, ERM® -


DA470k/IFCC (DA470K; Institute for Reference Materials and Management), has been produced. Binding Site assays have been shown to give accurate results when evaluated against this material and data was presented in a poster at AACC 2009, Chicago. Download your copy here.


SPAPLUS® Automated Specialist Protein Analyser DESCRIPTION PACK IgG SPAPLUS kit


Range 0.165-140, sensitivity 0.165 (g/L) IgG1 SPAPLUS kit


Range 0.15-144, sensitivity 0.15 (g/L) IgG2 SPAPLUS kit


Range 0.02-28, sensitivity 0.02 (g/L) IgG3 Latex SPAPLUS kit


Range 0.0055-4, sensitivity 0.0055 (g/L) IgG4 Latex SPAPLUS kit


Range 0.003-3.4, sensitivity 0.003 (g/L) Optilite®


Optimised Protein System See pages 7-9 for more information on SPAPLUS.


DESCRIPTION PACK IgG1 Optilite kit


Range 0.15-144, sensitivity 0.15 (g/L) IgG2 Optilite kit


Range 0.02-28, sensitivity 0.02 (g/L) IgG3 Latex Optilite kit


Range 0.0055-2.2, sensitivity 0.0055 (g/L) IgG4 Latex Optilite kit


Range 0.0043-64.8, sensitivity 0.0043 (g/L) See pages 2-5 for more information on Optilite. Units in brackets apply to both range and sensitivity. More information at: www.bindingsite.com 17 CODE


100 test NK006.OPT 100 test NK007.OPT


100 test LK008.OPT 100 test LK009.OPT


CODE


100 test NK004.S 100 test NK006.S 100 test NK007.S 100 test LK008.S 100 test LK009.S


Roche cobas® 6000 Analyser DESCRIPTION PACK IgG1 cobas 6000 kit


Range 0.33-60, sensitivity 0.33 (g/L) IgG2 cobas 6000 kit


Range 0.12-20 , sensitivity 0.12 (g/L) IgG3 Latex cobas 6000 kit


Range 0.014-4.375, sensitivity 0.014 (g/L) IgG4 Latex cobas 6000 kit


Range 0.018-2.7, sensitivity 0.018 (g/L) Hitachi Analysers DESCRIPTION PACK Hitachi COMBI kit


(Latex IgG3,4, non-latex G1, 2 & IgG) IgG-Range 0.26-65, sensitivity 0.26 (g/L) IgG1 Hitachi kit


Range 0.33-60, sensitivity 0.33 (g/L) IgG2 Hitachi kit


Range 0.12-20, sensitivity 0.12 (g/L) IgG3 Latex Hitachi kit


Range 0.007-4.375, sensitivity 0.007 (g/L) IgG4 Latex Hitachi kit


Range 0.009-2.7, sensitivity 0.009 (g/L) Supplied with parameters for use on:


Hitachi 911, Hitachi 912, Hitachi 917 & Roche/Hitachi Modular P. Detailed analyser programming information is available.


Beckman Analysers DESCRIPTION PACK IgG1 Beckman IMMAGE®


Range 0.15-40, sensitivity 0.15 (g/L) IgG2 Beckman IMMAGE®


kit kit


Range 0.0972-26.25, sensitivity 0.0972 (g/L) IgG3 Latex Beckman IMMAGE®


Range 0.01-2, sensitivity 0.01 (g/L) IgG4 Latex Beckman IMMAGE® Range 0.0073-1.5, sensitivity 0.0073 (g/L)


kit kit


58 test 58 test


58 test 58 test


CODE


NK006.IM NK007.IM


LK008.IM LK009.IM


The user is required to set up a user-defined reagent (UDR) for each assay. DESCRIPTION PACK IgG1 Beckman AU®


Range 0.15-80, sensitivity 0.15 (g/L) IgG2 Beckman AU®


Series kit Series kit


Range 0.0972-52.5, sensitivity 0.0972 (g/L) IgG3 Latex Beckman AU®


Range 0.0156-5, sensitivity 0.0156 (g/L) IgG4 Latex Beckman AU®


Range 0.004-3, sensitivity 0.004 (g/L) For use on Beckman AU® : 400, 480, 640, 680, 2700 and 5400.


A reaction accelerator for the latex assays is included. Manufacturing date to expiry is maximum 12 months.


Series kit Series kit


100 test 100 test


100 test 100 test


CODE


NK006.AU NK007.AU


LK008.AU LK009.AU


CODE 5x45 test LK001.H


4x45 test 4x45 test 4x45 test 4x45 test


NK006.H NK007.H LK008.H LK009.H


100 test 100 test 100 test 100 test


CODE


NK006.CB NK007.CB LK008.CB LK009.CB


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