CONTINUING EDUCATION TEST SARS-CoV-2 sequencing for public health impact
JUNE 2022 [This form may be photocopied. It is no longer valid for CEUs after December 31, 2023.] Passing scores of 70 percent or higher are eligible for 1 contact hour of P.A.C.E. credit.
TEST QUESTIONS Circles must be filled in, or test will not be graded. Shade circles like this: 1. What happens to a virus when proteins mutate?
{A. The virus can become more transmissible
{B. The virus becomes resistant to certain treatments
{C. The virus can gain the ability to evade antibody-mediated immunity.
{D. All of the above
2. Currently, which variant is the most prevalent SARS-CoV-2 variant of concern in the United States?
{A. Delta {B. Alpha
{C. Omicron {D. Beta
3. Where was the private SARS-CoV-2 clinical laboratory infrastructure and newly developed clinical research lab located?
{A. In two large counties in California {B. In a large suburb of New York {C. On a Louisiana college campus
{A. 100 {B. 200
{A. 30 {B. 10
Not like this:
8. During sequencing, what number of reads are aimed for, per sample?
{C. 1 to 2 million {D. 5000
9. Regarding the quality control of reads, a minimum quality score is________.
{C. 100 {D. 65
10. When mapping, paired-end reads were then mapped against the____________.
{A. Shanthi seafood market pneumonia virus isolate
{B. Shanghai beef processing plant viral isolate
{C. Guangzhou rice processing plant viral isolate
{D. In the heart of Los Angeles’ largest medical facility
4. What was one requirement for participation in the study?
{A. Participants must be below the age of 18
{C. Participants must have a positive SARS-CoV-2 RT-PCR result with a cycle
{D. All of the above { A. True
{A. 15 mL {B. 10 mL
threshold value less than or equal to 30 cycles within 5 days of enrollment and sample collection
5. Ideal subjects for the study include pregnant women and nursing home residents.
{ B. False
6. Specimens were placed into _________ collection tubes containing DNA/RNA Shield Stabilization Solution.
{C. 5 mL {D. 0.5 mL
7. How was RNA extraction performed using chaotropic agents/silica-based method(s)?
{A. mmanual silica column-based extraction method
{B. modified automated magnetic silica beads-based extraction method
{C. A and B {D. None of the above
{B. Participants must have reliable transportation to and from the laboratory testing site
15. What was the median age of the study’s participants?
{A. 17 {B. 13
{C. 43 {D. 20
16. According to the World Health Organization, a strong and resilient global sequencing network that provides useable and timely results is needed to ________________.
{A. maximize the public health impact
{C. help citizens find testing sites {D. None of the above
{D. Wuhan seafood market pneumonia virus isolate
11. What samples were used as control samples in order to validate the SARS-CoV-2 sequencing assay?
{A. Delta variant samples {B. Gamma variant samples {C. BEI SARS-CoV-2 samples {D. cDNA samples
12. Variable sites were generated, regardless of coverage depth assuming a haploid genome using bcftools (bcftools call -mv -Ov).
{A. True {B. False 13. Why did data need to undergo alignment?
{A. To identify gaps in generated consensus sequences
{B. To match them with positions in amino acid sequences carrying hallmark
{C. A and B {D. To rule out any environmental factors
mutations
14. Why were results submitted to Global Influenza Surveillance & Response System EpiCoV database, a public surveillance service?
{A. This step is mandated by law
{B. The database service is a safe, secure place to house the results
{C. For the safety of the laboratorians performing the tests
{D. For widespread data sharing and surveillance
Tests can be taken online or by mail. Easy registration and payment options are available through NIU by following the links found at
www.mlo-online.com/ce. PLEASE PRINT CLEARLY NAME CITY PHONE STATE ZIP MAILING ADDRESS INSTITUTION/FACILITY E-MAIL ADDRESS
Send your $20 check payable to Northern Illinois University with this form to: University Outreach Services, Northern Illinois University, DeKalb, IL 60115-2860 Phone: 815-753-0031 FEE NOT REFUNDABLE OR TRANSFERABLE
P = Poor; E = Excellent
1. To what extent did the article focus on or clarify the objectives?
P E
2. To what extent was the article well-organized and readable?
P E
3. How will you use the CE units? state license
recertification
employment other
CE Licensure Information for FL and CA:
FL: Your FL license number:____________________ (required for CE credit)
CA: Accrediting Agency: 0001 (for use in submitting your CE credits to CA)
MLO and Northern Illinois University (NIU), DeKalb, IL, are co-sponsors in offering continuing education units (CEUs) for this issue’s CE article. CEUs or contact hours are granted by the College of Health and Human Sciences at Northern Illinois University, which has been approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.®
program. Approval as a provider of continuing education programs has been granted by the
state of Florida (Provider No. JP0000496). Continuing education credits awarded for successful completion of this test are acceptable for the ASCP Board of Registry Continuing Competence Recognition Program. Readers who pass the test successfully (scoring 70% or higher) will receive a certificate for 1 contact hour of P.A.C.E.® credit. Participants should allow three to five weeks for receipt of certificate. The fee for this continuing education test is $20. This test was prepared by Amanda Voelker, MPH, MT(ASCP), MLS, Clinical Education Coordinator, School of Health Studies, Northern Illinois University, DeKalb, IL
16 JUNE 2022
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{B. allow labs to better schedule their daily testing
17. Why are faster identification of new SARS-CoV-2 variants of concern and understanding the rates in their change of prevalence needed?
{A. So, the mask mandate can be removed {B. So, large social gatherings can take place
{C. They could help parents make informed decisions about school children’s social distancing practices
{
D.They could be critical predictors of new waves of SARS-CoV-2
18. There are many reports of SARS-CoV-2 detected among many animal species, which may become reservoirs of infection and future spillover events. Which types of animal species are they referring to?
{A. Feral animals that roam wild {B. Those that closely interact with people {C. Air-born creatures {D. Fish and game wildlife
19. While effective treatments have been developed for SARS-CoV-2, what causes them to be underutilized?
{A. Treatments are costly {B. There is a lack of awareness of treatments {C. People are afraid of injections {D. A and B
20. What is one primary reason why access to COVID-19 testing continues to be a problem?
{A. People can’t find testing sites {B. People are afraid of injections {C. People are uninsured
{D. People don’t have reliable transportation to get to testing sites
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