Sample Preparation & Processing


CO2 incubator with built-in roller


Crafted with precision by Wiggens, the CO2 incubator emerges as essential tool for various experimental pursuits, from cultivating animal cells to hatching and germinating processes. With rapid heating, swift temperature recovery, and precise temperature control, this premium incubator ensures optimal


conditions for cell growth and research.


Its innovative design features electric heating wires surrounding the chamber, ensuring uniform heat distribution. With dry-wall insulation, maintenance is minimised while temperature stability is maximised. A base heater maintains water in a tray for humidity control, while a circulation fan ensures even moisture dispersion. Additionally, front door and frame heaters prevent condensation, maintaining clarity for observation.


Partnering seamlessly with the CO2 incubator, the Celrol Elite roller apparatus offers configurations from 5 to 88 bottle positions, facilitating cell attachment culture. This versatile apparatus accommodates standard plastic bottles, providing scalability and convenience for researchers. The roller culture technique,


utilising gentle rotation, enhances gas exchange and promotes cell growth.


For high-demand applications like vaccine production, the Celrol Elite can be expanded with additional layers, accommodating up to 11 tiers of bottles. Wiggens supports this system with options for battery backup, SMS alarm notifications, and direct printer connections for streamlined reporting. With its adaptability to standard cell culture bottles, the Celrol Elite roller apparatus offers a versatile solution for a wide array of biotechnological endeavours. More information online: ilmt.co/PL/zz7Q and ilmt.co/PL/pKbB


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Organic pollutant extraction in environmental samples: 12 application reports


The EPA test methods for evaluating solid waste (SW-846) provide a comprehensive source of information on sampling, sample preparation, analysis, and reporting. EPA 3546 was formally included in SW-846 and is a microwave assisted solvent extraction (MASE) procedure for extracting water insoluble or slightly water soluble organic compounds such as organochlorine pesticides, semivolatile organics, PAHs, PCBs, phenoxyacid herbicides, phenols, dioxins, and furans from soils, clays, sediments, sludges, and solid waste.


The above compounds were traditionally extracted using lengthy methods that required large volumes of solvents. MASE is a rapid sample preparation technique that enables efficient extractions but using reduced amounts of solvents with the ability of working at higher temperatures and pressures. For example, the Milestone Ethos X microwave extraction system from Analytix can extract the target compounds from environmental samples in full compliance with EPA 3546 (100-115 °C and 50-150 psi) and is capable of quickly processing up to 24 samples and 30 g of sample per vessel simultaneously thereby improving the limit of quantitation (LOQ) for analysis. This results in the capacity to extract over 200 samples in an 8-hour workday and the potential problems of contamination, memory effects, and cleaning are eliminated due to the use of disposable glass vials.


Twelve application reports highlighting the advantages of MASE for environmental samples are available from Analytix that demonstrate the recoveries of certified environmental standard materials being in compliance with the certificate values. In addition, it demonstrates that a wide range of different compounds can be completely extracted, even in large sample amounts.


The use of disposable glass vials coupled with speed of extraction and reduced solvent consumption makes the Ethos X a unique and innovative solution for the extraction of contaminants from environmental samples, providing unmatched ease of use and low running costs.


Contact Analytix for a copy of the application reports. More information online: ilmt.co/PL/85ep


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Compact solution for high-effi ciency peptide synthesis


Vapourtec has unveiled the Peptide-Builder, a compact and fully automated benchtop peptide synthesiser, at TIDES Europe in Hamburg. Designed to maximise efficiency and minimise solvent consumption, the Peptide-Builder integrates Vapourtec’s innovative Variable Bed Flow Reactor (VBFR) technology.


Engineered with customer feedback in mind, the Peptide-Builder offers the smallest high-throughput solution on the market, enabling the production of more peptides per square meter while conserving reagents. Its flexible scale accommodates synthesis ranging from 0.05 mmol to 1 mmol, making it compatible with all resin types.


The system operates sequentially, synthesising a GLP-1 analogue in under four hours and up to 16 analogues over a weekend. The intuitive software, developed in collaboration with leading pharmaceutical companies, allows users to simply input sequences and resin amounts, press ‘run,’ and let the system handle the rest.


Reagent consumption is reduced to less than three equivalents of amino acids, while solvent use is kept below 80 ml per mmol per cycle. The system enables rapid and efficient solvent exchange for automated side-chain addition. At the conclusion of synthesis, it provides detailed insights, including aggregation analysis and Fmoc removal quantification, in a comprehensive final report.


Dr Manuel Nuño of Vapourtec commented: “The VBFR technology transforms peptide synthesis by enhancing efficiency, streamlining workflows, and minimising space requirements.”


More information online: ilmt.co/PL/Vbnp 63676pr@reply-direct.com


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