epth sample analysis covering both fundamental and applied applications range of molecule sizes and classes. The illustrative examples presented int at the capability of the overall IM-MS instrument. This present nd ongoing developments ensure that the Cyclic IMS instrument will be at nt of the continued growth of IM-MS as an analytical technology for ample analysis.


Ubiquitin 6+


Not activated Activated


(a)


15 Mass Spectrometry required 100 passes (98 m separation length) around the device [2].


0 20 40 60 80 100 120 140 160 Arrival Time (bins)


1.0 ms Segment 180 200 (b)


The multifunction capability of the cyclic IMS is illustrated in Figure 3. Here the (M+6H)6+ charge state of bovine ubiquitin has been m/z selected for mobility separation. Figure 3(a) shows the single pass separation for the non-activated ions (blue trace) and ions activated prior to entry into the mobility separator (red trace). From these data it is apparent that, even without activation, there is more than a single structure (conformation) of the (M+6H)6+ species present. Further, it can be seen that activation changes the distribution of conformations. Figure 3(b) shows the sequence of a single pass separation with a 1ms segment of the non-activated ions being ejected (using the array) to the pre-array store, followed by a single pass separation of these ions following re-injection to the cyclic IMS. Figure 3(c) shows the single pass separation of both the non–activated segment (blue, re-scaled) and the segment activated on entry to the cyclic IMS (red). Interestingly, the activated segment species has an arrival time profile close to that of the activated species in Figure 3(a) but originates only from a sub-section of precursor ions. This implies that on activation, essentially all conformations become accessible to the selected precursor ions, rather than a subset defined by their original conformation.


Segment 1 pass


0 40 80 120 160 200 240 280 320 360 400 Arrival Time (Bins)


(c)


The advent of the cyclic IMS technology provides an incredibly powerful and flexible tool for in-depth sample analysis covering both fundamental and applied applications of a broad range of molecule sizes and classes. The illustrative examples presented here only hint at the capability of the overall IM-MS instrument. This present capability and ongoing developments ensure that the Cyclic IMS instrument will be at the forefront of the continued growth of IM-MS as an analytical technology for complex sample analysis.


References


1. Ujma, J.; Ropartz, D.; Giles, K.; Richardson, K.; Langridge, D.;Wildgoose, J.; Green, M.; Pringle, S., ‘Cyclic Ion Mobility Mass Spectrometry Distinguishes


Anomers and Open-Ring Forms of Pentasaccharides’ J. Am. Soc. Mass Spectrom. 2019, 30 (6), 1028−1037


2. Giles, K.; Ujma, J.; Wildgoose, J.; Pringle, S.; Richardson, K.; Langridge, D.; Green, M., ‘A Cyclic Ion Mobility-Mass Spectrometry System’ Anal. Chem. 2019, 91, 8564−8573


0 20 40 60 80 100 120 140 160 Aligned Arrival Time (Bins)


Arrival Time (arb units)


Figure 3. Arrival Time plots for Ubiquitin (M+6H)6+ (a) single pass separation (b) 1 ms segment selected from single pass ions then a single pass on the selected ions (IMS2) (c) expanded view of selected segment plot.


Read, Share and Comment on this Article, visit: www.labmate-online.com/article


Using Mass Spectrometry to Reveal the Thermal History of Recycled Polymers


BioChromato Inc has demonstrated how a Thermal Desorption and Pyrolysis/Direct Analysis in Real Time-Mass Spectrometry (TDP/DART-MS) analysis method can reveal the thermal history of recycled thermoplastic resins.


Recycling thermoplastic resins such as polypropylene is regarded as a vital step to help realise a more sustainable society. However, physical properties of thermoplastic resins such as strength and ability to elongate are known to degrade after repeated thermal reprocessing. It is there important to have analytical methods that can quickly and reliably reveal the thermal history of recycled polymers. Many traditional analytical techniques however fail to detect differences between virgin and recycled thermoplastic resins.


In this work, using an IonRocket sample introduction device, it was confirmed that TDP/DART-MS can be a useful way to evaluate thermal history, by using the antioxidants contained in thermoplastic resins as a ‘thermal history marker’.


The ionRocket from BioChromato is a temperature-heating device for direct thermal desorption and pyrolysis of samples, prior to ionisation and analysis by mass spectrometry. Using ionRocket a temperature gradient from ambient up to 600ºC can be achieved in just a few minutes. This enables the rmoplastic resins to be rapidly pyrolysed and then introduced into the DART-MS gas stream.


BioChromato has published a technical poster that shows how TDP/DART-MS, employing an ion-Rocket device, provides qualitative and quantitative information on changes in antioxidant additives incorporated into recycled polypropylene compared to virgin polymer pellets. This work shows the promise of TDP/DART-MS using an IonRocket device as a ‘go to’ analytical technique for analysing the longer-term durability of recycled polymers.


Read the technical poster: ilmt.co/PL/XZ1p More information online: ilmt.co/PL/9zjA


Powerful Solution for High Mass Analysis


Hiden Analytical is developing instruments for a new era of cluster analysis and implementation of nanoscale particles. The Hiden Cluster Series is a series of quadrupole MS systems, and components, developed specifically for high mass analysis that the analysis of nanoparticles requires. Systems include the 9 mm EPIC system, as well as the 20 mm DLS-20 system, for analysis of species up to 20,000 amu. Components include 9 mm and 20 mm pole diameter quadrupole assemblies with high-power RF operating at optimum frequency to accommodate high mass transmission required in cluster analysis.


Applications include nanoparticle analysis, molecular beam analysis, precursor and contaminant analysis for high mass species. Systems are equipped with Hiden’s multi-level software package, offering simple control of mass spectrometer parameters and complex manipulation of data and control of external devices.


Multiple sampling configurations are offered to suit research requirements, EPIC and IDP systems offer mid axis potential for negative ion detection and thermal desorption studies. The Cluster series are incorporated into the Plasma and SIMS analysis systems for plasma and surface characterisation. For pulsed deposition processes, time resolved measurements are offered to 50 ns time resolution.


The Cluster Series are designed to offer a fully featured, flexible and powerful solution for the monitoring of high mass species in RGA, Gas, Plasma and Surface analysis. More information online: ilmt.co/PL/pggB


57783pr@reply-direct.com 57818pr@reply-direct.com 180 200


Normalised Intensity


Normalised Intensity


Normalised Intensity


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