Drug Discovery, Pharmaceuticals & Cannabis Testing
iPSC culture techniques for drug discovery and therapeutics
AMSBIO has released a comprehensive online article highlighting the critical role of culture techniques in the use of induced pluripotent stem cells (iPSCs) for drug discovery and therapeutic applications. iPSCs represent an invaluable tool in these fields, but their effectiveness hinges on the ability to generate homogeneous cultures.
The article emphasises the significance of maintaining genetic stability, optimising culture conditions, and effectively managing single-cell expansion to ensure reproducibility and product quality. It discusses the use of chemically defined media, such as the advanced StemFit® product, which offers precise control over the cell growth environment, ensuring consistency across experiments. This consistency is essential for developing scalable protocols for iPSC culture and differentiation, which are crucial for clinical application.
Additionally, the article covers the vital role of cryopreservation in preserving iPSCs and organoids. It highlights the importance of using chemically defined, animal origin-free cryopreservation media like STEM-CELLBANKER® to maintain high yields and ensure the long-term viability of stem cells, even after extended storage.
Looking ahead, the article explores challenges in advancing 3D iPSC cultures, such as uncontrolled spheroid aggregation and cell shear stress. It introduces a novel approach using Laminin E8 fragments to create an effective extracellular matrix (ECM), fostering efficient stem cell production.
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Compact solution for high-effi ciency peptide synthesis
Vapourtec has unveiled the Peptide-Builder, a compact and fully automated benchtop peptide synthesiser, at TIDES Europe in Hamburg. Designed to maximise efficiency and minimise solvent consumption, the Peptide-Builder integrates Vapourtec’s innovative Variable Bed Flow Reactor (VBFR) technology.
Engineered with customer feedback in mind, the Peptide-Builder offers the smallest high-throughput solution on the market, enabling the production of more peptides per square meter while conserving reagents. Its flexible scale accommodates synthesis ranging from 0.05 mmol to 1 mmol, making it compatible with all resin types.
The system operates sequentially, synthesising a GLP-1 analogue in under four hours and up to 16 analogues over a weekend. The intuitive software, developed in collaboration with leading pharmaceutical companies, allows users to simply input sequences and resin amounts, press ‘run,’ and let the system handle the rest.
Reagent consumption is reduced to less than three equivalents of amino acids, while solvent use is kept below 80 ml per mmol per cycle. The system enables rapid and efficient solvent exchange for automated side-chain addition. At the conclusion of synthesis, it provides detailed insights, including aggregation analysis and Fmoc removal quantification, in a comprehensive final report.
Dr Manuel Nuño of Vapourtec commented: “The VBFR technology transforms peptide synthesis by enhancing efficiency, streamlining workflows, and minimising space requirements.”
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Specialist analytical products for the cannabis industry
The determination of the water content in cannabis is of great importance for the qualitative assessment of the active components (THC, CBD) as well as its storage stability. Karl Fischer titration offers a selective and reliable alternative to the drying method.
Compared to the reference method of national and international pharmacopoeias, which require a determination via the loss on drying over 24 hours, the Karl Fischer method provides a selective and reproducible result in less than 30 minutes with a significantly smaller sample quantity.
The results presented in Xylem’s white paper were obtained using SI Analytics® KF Titrators and KF Headspace Oven from Xylem using industrial hemp containing CBD.
Xylem offers several other analytical solutions to the cannabis industry, whether they be for licensed growers, or processors of substances containing cannabidiol (commonly known as CBD).
Cannabis growers should be mindful of temperature and humidity within the growing and storage areas as these parameters are also key for optimal growth, and both can be continually monitored using EBI 25 data loggers with remote alarm features.
In addition, growers can measure how well their plants are developing by using an Eclipse Brix refractometer to measure the sugar content of its sap, which is representative of a plant’s strength.
Processing cannabis forms part of the pharmaceutical, cosmetic, and more recently the food & beverage industry and requires stringent quality control. Amongst other tests, the measurement of optical rotation of cannabidiol by a polarimeter is simple and may be used to determine the purity or blend ratio of CBD type ((-)- or (+)- CBD) as well as used to ensure finished product is consistent between batches.
Download Xylem’s Cannabis Whitepaper today or visit Xylem’s website. More information online:
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