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Report on Nanoparticle Tracking Analysis use for Environmental and Biomedical Nanoparticle Monitoring


New Data Logger Aids Temperature Monitoring and Calibration A new and advanced data logger is the latest in a series of


technological developments to be unveiled by a leading medical refrigeration manufacturer, Lec Medical.


Suitable for pharmacies, hospitals, laboratories and clinics, this latest device comes complete with LCD screen and has been specifically designed to meet the increased demand for accurate temperature monitoring devices. It also boasts calibration capabilities.


Capable of measuring and storing up to 16,378 temperature readings over a -35 to +80°C (-31 to +176°F) range, this standalone data logger enables the user to easily set up the logging rate and start-time.


Stored data can then be downloaded by plugging the data logger into a PC’s USB port and running the purpose designed software under Windows 2000, XP, Vista, and 7. Data can be graphed, printed and exported to other applications.


A high contrast LCD displays a variety of temperature information, and at the touch of a button, the user can cycle between the current, maximum and minimum stored temperatures. In addition to user-programmable alarm thresholds, the data logger also enables immediate, delayed and push-to-start logging.


Lec Medical’s Brand Manager, Carolyn Holland commented: “We are delighted to offer an advanced data logger that is capable of calibration and is in keeping with MHRA guidelines for the manual and electronic measuring of recording devices. As well as the added benefit of an LCD screen, this latest device provides more comprehensive temperature logging and mapping.”


Carolyn added: “As with all Lec Medical products, the new data logger comes at an affordable price without compromising on design or performance.”


The data logger comes complete with a long-life lithium battery, which can typically allow logging for up to one year and it is protected against moisture to IP67 standard when the protective cap is fitted.


Ilya Kurochkin is Professor and Head of the Laboratory of Postgenomic Chemistry in the Department of Chemistry at the Lomonsov Moscow State University. His research requires characterisation of size distribution, concentration and to identify the presence of certain epitopes in two areas: exosomes for the prediction of adverse outcomes in patients with chronic heart failure and for the characterisation of gold, silver and metal oxide nanoparticles in the development of new electrochemical biosensors and nanobioanalytical systems based on nanoplasmonic structures.


In the research, it is important to be able to make a quick calculation of the number and estimate the size distribution of the exosomes in the biological fluids. The use of plasmonic nanoparticles will allow multiplex analysis of a large number of target proteins in exosomes. These measurements provide information about a specific protein composition of exosomes, and thus make it the basis of a new nanobioanalytical platform – ‘human exosome profile’.


To achieve this, Professor Kurochkin chose Nanoparticle Tracking Analysis, NTA, from NanoSight as his preferred technique as he needed accurate information on the size and number of his nanostructures in the presence of much larger particles. Prior to NTA, he used a combination of techniques including dynamic light scattering, atomic force microscopy and electron microscopy. As he noted, "The main benefit of the NTA is the ability to accurately measure size distribution for nanostructures in the presence of much larger particles directly in test solutions.


The fact that the intensity of light scattering is proportional to the particle diameter to the power of 6, DLS (which captures the signal from all the particles in the sample mixture) does not give an accurate measurement of a particle with a small diameter in the presence of particles with a larger diameter. NTA captures the trajectories of individual particles and therefore the ratio of the particle size does not affect the accuracy of the analysis.


Thus, the fact that NTA measures each particle separately is very important. I am particularly excited that I can measure my exosomes with specific antigens using fluorescent tags and plasmonic nanomarkers (gold or silver nanoparticles). Using NTA perfectly overcame the earlier experimental problems of my work. Measurements are rapid and by counting particle by particle, I achieved the level of accuracy I was seeking."


MICROSCOPY MORE INFO. 602


New Range of Pipette Controller Filters Available


Filter Devices are delighted to offer a range of pipette controller filters, ideal for use in laboratory and cleanroom environments where quality control of potential cross contamination is a high priority.


Using their expertise in producing custom made in-line filtration devices, their new


24mm pipette controller filters contain a 0.2ųm or 0.45ųm hydrophobic membrane accommodated in a housing tested to exacting standards. Ensuring high performance for the user and a quality delivered product every time, Helapet provide batch coding for full traceability.


Offered with Male Slip Luer connectors, the new 24mm Pipette Controller filters can securely fit into a majority of pipette controller models. Filter Devices also offer its quality Varidisk®


service allowing the flexibility to respond quickly, and


hold stock for next day delivery. Whether you wish to have OEM filters individually custom printed with your brand, individually or bulk packed, Filter Devices can provide the best solution.


LAB PRODUCTS MORE INFO. 601 LAB PRODUCTS MORE INFO. 599


Multimode Reader Flexibility Extended with Launch of new Module Tecan has developed an AlphaScreen®


Infinite®


module for its popular cost-effective solution for AlphaScreen and AlphaLISA®


F200 PRO multimode reader, providing customers with a assays.


Offering excellent sensitivity for screening and research, the new option further extends the flexibility of the Infinite F200 PRO for low and medium throughput applications.


The AlphaScreen module combines the Infinite F200 PRO’s established fluorescence top optics with a bespoke filter set designed to use the instrument’s standard flashlamp.


This set-up ensures optimal assay performance without the need for a specialised reader, complementing the system’s absorbance, fluorescence intensity, luminescence, fluorescence polarisation and time-resolved fluorescence resonance energy transfer measurement modes.


LAB PRODUCTS MORE INFO. 600


LABMATE UK & IRELAND - JANUARY 2013


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