25 Tailored Automation of PCR Setup and Nucleic Acid Purification
Two new additions to Eppendorf’s extensive range of automated pipetting systems are uniquely tailored for PCR setup and nucleic acid purification, yet retain the flexibility that allows their use as open systems for diverse automated liquid handling applications. The new epMotion®
intensive pipetting tasks, saving time and improving the reliability and reproducibility of results.
The epMotion M5073 fully automates the process of DNA purification, providing reproducibility with high yield and purity. Setup time is short and elution volumes as low as 25µL for high concentrations can be used. Eppendorf’s MagSep reagent kits are specifically designed for use with the epMotion M5073. These ready to use reagents, supplied in a specific tray, eliminate the need for manual buffer handling and require only room temperature storage.
Per run 1 to 24 samples can be extracted for nucleic acid from a variety of sources, such as blood, tissue, cells, bacteria or viruses. The epMotion P5073 helps streamline and automate the whole PCR setup workflow in 96 or 384 well format. Four different, step-by-step guided PCR assistants are available to facilitate working with normalisation, dilution, setup reactions and composing mastermixes. Both the epMotion P5073 and epMotion M5073 have 6 SBS positions and are installed with a large colour touch screen for optimum ease-of-use. An Eppendorf CleanCap option minimises contamination risks with the use of a HEPA filter and UV lamp.
Circle no. 595
Innovative Peristaltic Pump Provides Three Independent Channels from a Single Pump
New Electrophoresis System Enables Real-Time Size Fractionation
Now you can recover your DNA in real-time, as your gel runs, with the new DNA-VIEW Electrophoresis System from Alpha Laboratories Ltd. This versatile, portable, self contained horizontal gel electrophoresis system has an adapted lid, containing an orange spectral emission filter that lets you see your samples separate in real time. You can harvest the DNA of interest as soon as it enters the ‘extraction’ tier.
Combining the flexible, high resolution capabilities of the Clarit-E ® CHOICE system, with an integrated power supply and blue LED gel illuminator, DNA-VIEW provides a single convenient, time and space saving compact bench top unit.
The efficiency of DNA recovery from Ethidium Bromide and SYBR stained gels is maximised and the number of steps involved in post electrophoretic purification are reduced.
DNA-VIEW uses safe blue light to track DNA, without the risk of harmful UV causing mutagenesis or compromising cloning.
A new design of peristaltic pump which allows independent flow control of three separate channels within a single pump has been introduced by IDEX Health & Science. Available in the UK from Michael Smith Engineers the Ismatec®
Reglo ICC
(independent Channel Control) peristaltic pump expands the power of laboratory and life-science pumping by providing individual control of each fluidic channel and so eliminates the need for multiple pumps.
Maximising available bench-top space is important for many laboratories and the Reglo ICC pump helps to minimise the clutter of multiple pumps as well as allowing users to solve application complexity in a single pump.
The key features and benefits include, continuous pumping or precision dispensing, in one, two, or three channels, the flexibility of bi-directional flow in each channel and easy-to-use tubing cassettes for quick, convenient changeovers. Most importantly, independent channel calibration minimises the tube- to-tube differences and this results in optimum accuracy for a multi-channel peristaltic pump.
The Reglo ICC pump, which is self-priming and is not sensitive to dry-running, can handle flow rates from of 0.002 up to 35 ml/min and differential pressures up to 1 bar, while an easy-to-use USB interface means connections can be made quickly.
Circle no. 597
Ultra-High Throughput Microwave Workstation Added to Sample Preparation Range
The new MASTER 70-vessel ultra-high throughput closed microwave digestion/extraction workstation, produced by SINEO and distributed to the UK by Analysco, delivers efficient and effective microwave digestion of difficult samples in preparation for ICP analysis. Capable of processing up to 70 sample vessels simultaneously, the instrument is well suited to high-throughput laboratories whatever the application. The MASTER series works efficiently with pressures of up to 4MPa and at temperatures up to 250°C, combining precise temperature control and monitoring with ease of use and user safety.
The MASTER series is designed to work seamlessly with vessels and rotors of different quantity, temperature and pressure bearing capacities. Users can choose to load 15, 18, 40 or 70 vessels, each of 70ml capacity, during each run. The rotation of the high throughput vessel frame means that all the samples are heated evenly across the microwave field. A flexible trolley allows a full rotary table to be loaded without the user needing to touch the vessel itself, which might be pressurised and at very high temperature.
During heating, automatic feedback provides researchers with updates on output power, reaction temperatures and pressure. Along with continuous non- pulse microwave heating, this ensures both instrument safety and efficiency, further secured by a pressure relief blow out disk, which keeps the vessel sealed while in use.
Circle no. 598
This new system saves on time-consuming gel elution techniques. Once harvested, the DNA only needs to be cleaned by a straightforward ethanol precipitation. It works with standard Ethidium Bromide, SYBR Green and SYBR Safe gels, cast within the 15x7, 15x10 or 15x15cm Clarit-E CHOICE gel trays. It does not require expensive precast gels and accessories.
Circle no. 596 P5073 and M5073 workstations automate and simplify what are traditionally complex, labour-
The Spotlight could be on you!
Contact Gwyneth Astles on +44 (0)1727 855574 or email:
gwyneth@intlabmate.com
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