7 ASTM Publishes E2834 Standard Defining Nanoparticle Tracking Analysis
NanoSight is delighted to welcome publication of American Society of Testing Materials ASTM E2834 - Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA). This document details application of Nanoparticle Tracking Analysis (NTA) to measurement of particle size distributions for suspended particles from ~10nm to the onset of sedimentation. Duncan Griffiths, NanoSight's USA West Sales Manager commented: "This document has taken almost three years to develop. It provides a rigorous review of the core science driving Brownian motion and the derived parameters of mean, mode, percentile values and concentration. The scrutiny of demanding third-party experts has been fundamental in producing a thorough appraisal of NTA. The result is a meticulous presentation of science and methodology. We are delighted." The UK's National Physical Laboratory (NPL) has seen NanoSight develop NTA since its early days more than five years ago. Helen Sharman from NPL commented: "NPL has been working on the characterisation of nanomaterials for many years, in-part collaborating with NanoSight. The nanomaterials team has followed the development of the NTA technique with great interest due to its unique capability in the area of nanometrology. As the UK's national measurement institute, NPL recognises the importance of this standard and the capability of the NanoSight instrumentation to provide consistent, precise and comparable data."
NanoSight CEO Jeremy Warren added: "Whilst this standard is a milestone, it is also a building block. Many NanoSight users have requested this definition of the technique as they present data in research papers and to regulatory bodies for product approvals and clinical trials. We will now move to provide industry with test methods for specific materials to help lock down SOPs. The timing of this publication is helpful as the European Commission move to consider methodologies to address the characterisation challenges of their recently-published definition of nanomaterials. Here we see a significant role for NTA's unique nanoparticle counting capability". The Standard is available from ASTM at
www.astm.org/Standards/E2834.htm
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Dynamic Light Scattering System used for Measuring Protein Structural Transition
Using the Zetasizer µV dynamic light scattering (DLS) system from Malvern Instruments, Professor Mario L. Calcagno and his team at the Biochemistry Department of the Faculty of Medicine, Universidad Nacional Autónoma de México (UNAM, the National Autonomous University of Mexico) have been able to distinguish allosteric transition [structural rearrangements] in a single E. coli protein. Characterising protein allostery is challenging because of its sensitivity to experimental conditions, however, a quantitative description of allosteric transition is important in understanding and controlling metabolic and other biochemical processes.
“We use the Zetasizer µV to characterise the size of proteins and how they interact to produce multimeric forms or even supramolecular arrangements of the protein such as viral capsids [shells],” said Dr Ismael Bustos-Jaimes, from Laboratory of Physical Chemistry and Protein Engineering at UNAM commented “The Zetasizer µV allows us to work with sizes in the range of 1 nm to 500 nm and follow each assembly and stability parameter, such as pH, temperature and ionic strength, guiding us to the optimal production conditions for these virus-like particles. The sensitivity of the Zetasizer µV has additionally allowed my colleague, Prof Calcagno, to analyse allosteric transitions” explained Dr Bustos-Jaimes. “The size of the hexameric glucosamine-6-phosphate deaminase protein from E. coli changes its shape to a more compact form upon allosteric-activator binding, and this change can be measured. The Zetasizer µV software is user-friendly and in addition to measuring particle size it delivers information about the quality of sample preparation. This is very important when you work with molecules which are prone to uncontrolled aggregation,” said Dr Bustos-Jaimes.
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Filtration Society’s 2012 Conference Programme Announced
Whitehouse Scientific is once again a major sponsor of The Filtration Society’s annual conference and exhibition, which returns on 2-3 October 2012 at the Mercure Chester East Hotel, Christleton, Chester, UK. The main one-day event this year focuses on ‘Filter Characterisation’ and will be preceded by a Filter Testing Short Course.
Dr Graham Rideal, CEO of Whitehouse Scientific is a former chairman of The Filtration Society
and currently its science correspondent. “Whitehouse Scientific is delighted to again support the annual Filtration Society conference. One of its attractions for me, is the way that it gives conferees the opportunity to raise matters related to filter testing that are of particular interest to them, thus enabling us to learn from one another.”
The Filtration Society conference always attracts industry leaders and this year is no exception. On day one, short course presenters will be Mark Crooks (TSI, UK), Danny Pattyn (
Porometer.com, Belgium), Dr Christophe Peuchot (IFTS, France), Dr Steve Tarleton (Loughborough University, UK) and Professor Richard Wakeman (Consultant, UK). Topics covered will include particle size and shape analysis, porometry and characterising and testing filter media.
On day two, the main ‘Filter Characterisation’ event will include expert presentations on topics as diverse as air filter testing, planning lab scale filtration tests and the importance of particle statistics in challenge testing.
Attendees will also be able to see the latest filtration products at the trade exhibition, which features the unique ‘Technology burst’ session in which exhibitors are given the opportunity to make 5-10 minute presentations about their products or services.
Full details of the conference programme, short course and exhibition, together with registration information, are available on the Filtration Society’s website at
www.filtsoc.org.
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