16 Good Pipetting Practice Ensures the Success of your Research
Pipettes are an essential tool in life science research as the majority of experiments require the careful handling of precise volumes of liquid. Their accuracy and precision is therefore vital for producing good quality results.
To ensure the success of your research you need a good understanding of your liquid handling workflows and the equipment you need for each task. You need to consider how well your pipettes are maintained and what impact this could have, as well as your pipetting technique and if you are at risk of Repetitive Strain Injuries (RSI).
To help and advise you, Anachem with Rainin and Mettler Toledo has created Good Pipetting Practice (GPP). GPP is a specially developed five step programme comprised of risk analyses, educational materials and seminars. The series is designed to help you understand and reduce the risks involved in your workflow process for liquid handling and for you to improve the consistency of your results.
By implementing a Good Pipetting Practice programme in your laboratory everyone on your team will learn how to: carefully choose their pipetting instruments; optimise their liquid handling workflows; use the correct technique; recognise the risk associated with out-of- calibration pipettes and the role of routine checks.
A good place to start is
www.mt.com/gpp where you can find out what your pipetting risk is with the Free GPP Risk Check tool. It is a confidential online tool that takes just five minutes to complete. Upon completion, a link is offered for a detailed analysis of your pipetting risks and practical tips on how to minimise them.
A specially-written white paper, ‘The Effect of Pipetting on Experiment Outcome’, examines scientific papers that have shown how pipetting errors affect experimental results and the importance of routine checks and proper maintenance.
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The LMS Series ‘1A’ Cooled Incubator is a reliable and accurate Temperature controlled cabinet (-10°C to +50°C) designed for optimum performance and reliability. The design of the purpose built incubator facilitates the addition of optional extras to provide greater flexibility for individual use, making it suitable for a wide range of applications.
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The LMS Series Three Cooled Incubator is a reliable and accurate temperature controlled cabinet (-10°C to +50°C) which has proved its worth over many years in laboratories both in the U.K. and overseas.
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ACE Excel 2µm Ultra Robust UHPLC columns from Advanced Chromatography Technologies offer you all the benefits that chromatographers have come to expect from ACE columns (exceptional reproducibility and robustness, combined with excellent quality and efficiency) but have been especially engineered to withstand the demanding conditions of UHPLC.
Additionally, ACE Excel UHPLC columns are available in a wide range of selectivities, including the unique ACE C18-AR and ACE C18-PFP phases, enabling chromatographers to take advantage of the extremely powerful benefit of selectivity combined with efficiency. ACE Excel 2µm columns are also available with C18, C8, C4, CN, AQ, Phenyl and Silica phases.
ACE Excel Ultra Robust UHPLC columns are packed with the same ‘Ultra Inert’ ACE particles that have set industry standards for purity and efficiency, resulting in a range of UHPLC columns that are at last truly scalable with larger particle HPLC columns. ACE Excel UHPLC columns are compatible with all UHPLC and HPLC systems and are robust up to 1,000bar (15,000psi) and temperatures up to 100°C. Advanced Chromatography Technologies also offer a range of UHPLC reusable column connectors (P/n EXL- CC) which have been specifically designed for UHPLC and are suitable for use with 1/16" OD tubing and virtually all manufacturers’ UHPLC systems and all brands of UHPLC columns. These compact, one-piece design fittings are simple and easy to use. Installed correctly these fittings are non-permanent swaging, protecting your valuable time and equipment. They are reusable and will provide approximately 10 make and break cycles.
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